小花鬼针草中的苯丙苷类成分及抑制组胺释放活性  

Phenolic glucosides from Bidens parviflora and their anti-histamine activities

作　　者：王珏[1,2] 王乃利[1] 姚新生[1] 北中■ 

机构地区：[1]沈阳药科大学天然药化教研室,辽宁沈阳110016 [2]日本大学药学部生药学研究室,日本千叶船桥274-8555

出　　处：《中草药》2007年第5期647-649,共3页Chinese Traditional and Herbal Drugs

摘　　要：目的研究小花鬼针草Bidens parviflora全株的化学成分，并通过组胺抑制实验寻找活性成分。方法采用硅胶、Sephadex LH-20和ODS柱色谱分离化合物，运用1D NMR、2D NMR波谱学方法鉴定了化合物的结构，通过组胺抑制实验测定化合物抗过敏活性。结果分离鉴定了4个苯丙苷类及1个苯甲醇苷成分：4-羟基-3-甲氧基苯丙三醇8-O-β-D-葡萄糖苷（guaiacyl glycerol 8-O—β—D—glucoside Ⅰ）、丁香酚苷（syringin，Ⅱ）、4-烯丙基-2-甲氧基苯酚-O-（6-O-β-D-芹糖基）-β-D-葡萄糖苷[4-allyl-2-methoxyphenol-O-（6-O—β—D—apiofuranosyl）-β-D-glucoside，Ⅲ]、5，7-二羟基色原酮-7-O—β—D-葡萄糖苷（5，7-dihydroxy chromone 7-O-β-D—glucoside，Ⅳ）、苄醇-O-β-D-葡萄糖苷（benzyl alcohol—O—β—D—glucoside，Ⅴ）。化合物Ⅰ～Ⅴ抑制组胺释放，IC50分别为70、61、〉100、52、〉100μg／mL。结论化合物Ⅰ～Ⅴ首次从本植物中分得，Ⅲ为未见文献报道的新化合物，命名为鬼针草酚葡萄糖苷（bidenphenol glucoside）。化合物Ⅰ～Ⅴ具有抑制组胺释放的活性。Objective Based on the activities against histamine release of Bidens parviflora to find biological active compounds. Methods The chemical constituents from B. parviflora were isolated by silica gel column chromatography and Sephadex LH-20 column chromatography, then purified by preparative HPLC. Their chemical structures were identified by 1D NMR, 2D NMR methods. Results Five phenolic glucosides were identified as guaiacyl glycerol 8-O-β-D-glucoside （Ⅰ）, syringin （Ⅱ）, 4-allyl-2-methoxyphenol-O-（6-O-β-D-apiofuranosyl）-β-D-glucoside （Ⅲ）, 5, 7-dihydroxy chromone 7-O-β-D-glucoside （Ⅳ）, benzyl alcohol-O-β-D-glucoside （ Ⅴ ）. Compounds Ⅰ - Ⅴ could inhibit the histamine releasing and their IC50 were 70, 61, 〉100, 52, 〉100 μg/mL, respectively. Conclusion Compounds Ⅰ - Ⅴ are isolated from B. parviflora for the first time, Ⅲ is a new phenolic glucoside named as bidenphenol glucoside. Compounds Ⅰ - Ⅴ exhibit the activities of anti-histamine release from rat mast cell stimulated by antigen-antibody reaction.

关 键 词：小花鬼针草 4-羟基-3-甲氧基苯丙三醇8-O-β-D-葡萄糖苷 4-烯丙基-2-甲氧基苯酚-O-(6-O-β-D-芹糖基)-β-D-葡萄糖苷(鬼针草酚葡萄糖苷) 丁香酚苷 组胺抑制活性 

分 类 号：R284.1[医药卫生—中药学]