兔超排及SPF桑椹胚超低温保存的研究  

Study on Superovulation and Cryopreservation of SPF Rabbit Embryos

作　　者：柴世庆[1] 柴家前[1] 王长江[2] 刘丽均[2] 郁丽丽[2] 徐平[2] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]中国科学院上海试验动物中心,201615

出　　处：《家畜生态学报》2007年第2期79-81,F0003,共4页Journal of Domestic Animal Ecology

基　　金：上海市科研计划项目;编号:034909007

摘　　要：采用EFS40、EFS20、mEFS40、mEFS20冷冻液对供试兔桑椹胚的冷冻保存进行了探索,旨在提高冷冻胚胎的复苏率。选取三个方案进行试验。结果表明:三个方案的复苏率分别为17.1%、69.6%和36.1%。孵化率分别为12.9%、60.9%、18.4%,方案二的复苏率和孵化率显著高于方案一、三(P<0.05),通过显微镜观测也证实方案二的冷冻胚胎的形态恢复良好。试验证明使用方案二对供试兔胚胎冷冻可以保持较高的复苏率。EFS40,EFS20 and mEFS40,mEFS20 solutions were used to cryopreserve the rabbit morulae to improve the anabiosis rate. The experiment was carried out with three schemes. The results showed that the anabiosis rate was 17.1%,69.6% and 36.1%,and the incubate embrgos rate was 12.99%,60.99%, 18.49% in three sehemes,respeetively. The result in second scheme eomparaed with the others was useful for keeping high anabiosis rate after eryopreservation.

关 键 词：EFS40 EFS20 兔 冷冻复苏 桑椹胚 

分 类 号：S811.5[农业科学—畜牧学]