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机构地区:[1]中国医科大学附属盛京医院,沈阳市110004 [2]中国医科大学,沈阳市110001
出 处:《实用医学杂志》2007年第9期1292-1294,共3页The Journal of Practical Medicine
摘 要:目的:探讨全身炎症反应综合征(SIRS)时大鼠心肌G蛋白的变化,为临床防治SIRS和多脏器功能障碍综合征的发生、发展提供理论依据。方法:Wistar大鼠24只,随机分为内毒素(LPS)注射后3h组(3.0h组)、LPS注射后6h组(6.0h组)、正常对照组,每组8只。实验组大鼠给予大肠杆菌内毒素(E.ColiO55B5,美国Sigma公司产品)20mg/kg腹腔注射,制造SIRS大鼠模型,免疫印记法测定大鼠心肌G蛋白的含量。观察3h、6h后SIRS大鼠心肌G蛋白的变化,并与正常对照组比较。结果:与正常对照组相比,LPS 3.0h组、LPS 6.0h组大鼠心肌Gsα蛋白的相对含量(单位:%)分别下降为92.375±2.187、89.755±4.621,P<0.01,尤以6.0h组下降明显;而LPS 3.0h组、LPS 6.0h组大鼠心肌Gi α蛋白含量均升高,分别上升为106.0±3.381(P<0.05)、111.0±6.590(P<0.01),尤以6.0h组升高明显。结论:SIRS大鼠心肌Gs、Gi蛋白发生改变,并且随着病情的进展变化明显,推测SIRS时心功能障碍的发生可能与G蛋白的异常变化有密切的关系。Objective To provide the theoretical support for clinical prevention and treatment of systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction syndrome through exploring the alterations of myocardial G protein in SIRS rats. Methods Twenty-four Wistar rats were equally randomized to LPS 3.0 h group, LPS 6.0 h group, or control group. A rat model of SIRS was established by an abdominal injection of LPS at a dose of 20 mg/kg. The contents of Gs and Gi protein were detected by Western blot 3 and 6 hours after the injection, respectively. Results Compared with that in the control group, the content of Gs was reduced to (92.375±2.187)% in LPS 3.0 h group,and to (89.755±4.621)% in LPS 6.0 h group ( P 〈 0.01) with a more obvious reduction in the latter one, whereas the content of Gi was elevated to (106.0 ± 3.381)% in LPS 3.0 h group(P 〈 0.05) and to (111.0 ± 6.590)% in LPS 6.0 h group ( P 〈 0.01) with a more significant elevation in the latter one. Conclusion Alterations of Gs and Gi protein occur in SIRS rats and differ significantly with the progress of the disease, which suggests that the pathogenesis of cardiac dysfunction in SIRS may be closely related to the abnormal changes in myocardial G protein.
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