丝裂原蛋白激酶在糖尿病慢性血管并发症中的作用  

The effect of MAPK on the chronic vascular complication of type 2 diabetics

作　　者：李新荣[1] 高景燕[1] 邓湘蕾[1] 李艳平[1] 

机构地区：[1]东南大学丁家桥校区基础医学院生化教研室,南京210009

出　　处：《中国糖尿病杂志》2007年第4期228-230,共3页Chinese Journal of Diabetes

摘　　要：目的研究在高糖条件下人血管平滑肌细胞丝裂原蛋白激酶(MAPK)活性、c-fos表达与糖尿病慢性血管并发症的发病机制的关系。方法新生儿脐动脉原代培养获得人血管平滑肌细胞。高糖为刺激因素,PD98059为MAPK特异性抑制剂,甘露醇作渗透压对照。同位素示踪法测定MAPK活性,免疫细胞化学方法检测c-fos表达。结果高糖条件培养下人血管平滑肌细胞内MAPK活性升高(77358±1822)、与对照组(47830±1946)相比差异有统计学意义(P<0.05)。高糖条件培养下人血管平滑肌细胞c-fos表达增加(0.314±0.058),与对照组(0.245±0.049)相比差异有统计学意义(P<0.05)。PD98059可阻止高糖诱导的c-fos表达。结论高糖可增加人血管平滑肌细胞MAPK活性及c-fos的表达,MAPK活性的增高与c-fos表达呈正相关。Objective To investigate the activity of MAPK and the expression of c-fos in human vascular smooth muscle cells cultured in high glucose and the mechanism of chronic vascular complication of type 2 diabetes. Methods The human smooth muscle cells were obtained by primary cell culture. They were stimulated by high glucose. PD98059 was a special inhibitor of MAPK. The MAPK activity was measured by liquid scintillation counting. The expression of c-fos was measured by immunochemistry. Results Compared with the control group （47830±1946）, the high glucose increased the activity of MAPK （77358±1822） （P〈0. 05）. Compared with the control group （0. 245±0. 049）, the expression of c-los increased （0. 314 ± 0. 058） （P〈0.05）. PD98059 inhibited the activity of MAPK. Conclusions The activity of MAPK and the expression of c-fos are increased when cells were cultured in high glucose. The activity of MAPK affects the expression of c-fos.

关 键 词：丝裂原活化蛋白激酶 c—fos血管平滑肌细胞 PD98059 

分 类 号：R587.2[医药卫生—内分泌]