聚结过滤法测定β-榄香烯固体脂质纳米粒的包封率  

Determination of the entrapment efficiency of β-elemene solid lipid nanoparticles by method of coagulation-filtration

作　　者：王艳芝[1] 郑甲信[1] 胡会国[2] 邓意辉[2] 毕殿洲[2] 孙伟[2] 

机构地区：[1]郑州大学药学院,河南郑州450001 [2]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2007年第5期271-274,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立聚结过滤法测定β-榄香烯固体脂质纳米粒(SLN)的包封率。方法先以体积分数为36%的乙醇水溶液溶解SLN制剂中的游离药物,然后用氯化钠水溶液聚结SLN并过微孔滤膜,以HPLC法测定滤液中β-榄香烯含量,计算包封率。结果该方法可将游离药物与SLN分开,平均回收率为97.4%,测得的β-榄香烯SLN的包封率为96.0%。结论使用聚结过滤法可准确、快速地分离出游离β-榄香烯,可用于该制剂包封率的测定。Objective To determine the entrapment efficiency of β-elemene solid lipid nanoparticle（SLN） by method of coagulation-filtration. Methods After consequently addition of agueous alcohol solution and agueous sodium chloride to the SLN, the dispersion was filtered and the solution containing free drug was obtained, content of β-elemene was determined by HPLC. Results Under the established condition, free β-elemene could be well separated from the preparation. The average recovery of β-elemene was 97.4 %. The entrapment efficiency of β-elemene SLN was （96.0 ± 0.41） %. Conclusions The method is quick and accurate to obtain free β-elemene for the determination of entrapment efficiency of β-elemene SLN.

关 键 词：Β-榄香烯 固体脂质纳米粒 包封率 聚结过滤法 

分 类 号：R94[医药卫生—药剂学]