8种卷柏属药用植物的RAPD分析  

RAPD Analysis of 8 Medicinal Species of Selaginella

作　　者：李娟[1] 万定荣[1] 陈科力[1] 

机构地区：[1]湖北中医学院药学系,湖北武汉430061

出　　处：《中药材》2007年第4期403-406,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金项目(30470193)

摘　　要：目的:用分子标记技术鉴别8种卷柏属药用植物并进行亲缘关系分析。方法:从60个随机引物中筛选出8个引物,采用随机引物扩增多态DNA(RAPD)技术,对8种卷柏属植物的17份样品的DNA提取物进行扩增和分析。结果:共扩增出58个位点,获得清晰的RAPD图谱,建立了8种卷柏属植物类群亲缘关系的树系结构图。结论:该方法显示了8种卷柏属植物之间明显的种间差异,以及同一种植物在不同产地产生的变异,能为这些植物种以及种下的分类鉴定提供遗传学依据。Objective ： To identify 8 medicinal species of Selaginella with method of molecular marker and to explore their genetic relationship. Methods： 8 primers were selected from 60 random primers to amplify the total DNA extracts of 17 samples with RAPD method and then the amplified were analyzed. Results： A total of amplified 58 bands and RAPD map were obtained. Based on them,a Jaccard＇s genetic similarity matrix and a dendrogram for the 8 plants were established using SPSS 11.5 software. Conclusion： The method revealed distinct differences of different species and the variation of the same species of Selaginella from different habitats. It can provide with genetic proof for authentication of some species and units below species.

关 键 词：卷柏 RAPD分析 分类鉴定 亲缘关系 

分 类 号：R282.5[医药卫生—中药学]