丹参对大鼠急性肺损伤的保护作用  

Protection Effect of Salvia miltiorrhiza on Model of Acute Lung Injury in Rats

作　　者：李敏[1] 李锋[1] 胡波[1] 王文[1] 任秦友[2] 行利[1] 张雅萍[2] 

机构地区：[1]第四军医大学西京医院 [2]第四军医大学唐都医院,陕西西安710032

出　　处：《中药材》2007年第4期442-444,共3页Journal of Chinese Medicinal Materials

基　　金：西京医院创新基金(XJCX05M29)

摘　　要：目的:通过观察丹参(SM)对急性肺损伤(ALI)大鼠肺水通道蛋白-1(AQP-1)的影响,探讨其保护作用。方法:SD大鼠48只,随机分为正常组、丹参注射液预保护组、丹参注射液7 d治疗组、ALI 7 d模型组、丹参注射液8hr治疗组、ALI 8 hr模型组,每组各8只。分别于7 d和8 hr后电镜HE染色观察各组大鼠肺组织病理改变,肺湿/干重比(W/D)观察肺脏水通透性的变化,免疫组化法及肺组织匀浆后Western blotting法检测AQP-1表达变化。结果:模型组均出现肺损伤症状。SM7 d治疗组的肺水肿病理改变与ALI 7 d模型组比较明显减轻,但两组AQP-1表达均与正常组无明显差异(P<0.05)。SM8 hr治疗组和SM预保护组与ALI 8 hr模型组比较肺水肿病理改变明显减轻,血流变明显改善(P<0.05),其中SM预保护组AQP-1表达上调明显,有显著差异(P<0.01)。结论:ALI时SM能增加肺组织毛细血管上AQP-1的表达,从而减轻其减少引起的肺水肿,在一定程度上缓解ALI病变的发生与发展。Objective ： To investigate the effect of SM on aquaporin-1 （ AQP1 ） expression in rats with acute lung injury （ ALI）, and study protection against ALI Methods ： 48 SD rats were randomly divided into normal group, ALI 7-day group, ALI 8-hour group, SM prevention group, SM 7-day treatment group, and SM 8-hour treatment group. At 8 hours and 7 days after treatment, lung wetto-dry weight ratio, lung pathology, electron microscope, AQP1 immunohistochemistry, and AQP1 Western blot were performed. Results： The model group rats all appeared ALI symptom. Compared with the 7-day ALI group, the degree of lung edema statement alleviated in SM 7-day treatment group. But the expression of AQP-1 was not significant between the two groups （P 〈 0. 05 ）. Compared with the 8- hour ALI group, the degree of lung edema statement alleviated in SM 8-hour treatment group and SM prevention group. The expression of AQP-1 was significantly expression in the group of SM prevention group （ P 〈 0. 01 ）. Conclusion ： SM may improve the body fluid metabolism and alleviate the degree of the lung edema by regulating the expression of AQP-1. The results suggest SM can regulate the activity of AQP-1 to improve the water metabolism of ALI.

关 键 词：丹参 急性肺损伤 水通道蛋白 保护作用 

分 类 号：R285.5[医药卫生—中药学]