碱性成纤维细胞生长因子在COPD大鼠模型气道重塑中的作用  

Potential role of bFGF in airway remodeling of chronic obstructive pulmonary disease rat model

作　　者：叶安霞[1] 谢正福[2] 赵华[2] 黄巨恩[3] 施焕中[2] 

机构地区：[1]柳州市人民医院呼吸内科,广西柳州545001 [2]广西医科大学第一附属医院,广西南宁530021 [3]广西医科大学组胚教研室,广西南宁530021

出　　处：《中国实用内科杂志》2007年第10期783-786,共4页Chinese Journal of Practical Internal Medicine

基　　金：广西壮族自治区卫生厅重点科研基金(桂卫重200003)

摘　　要：目的通过构建慢性阻塞性肺疾病(COPD)大鼠模型,探讨碱性成纤维细胞生长因子(bFGF)在COPD气道重塑的作用和可能机制。方法2005年7月至8月,于广西医科大学动物实验中心,将40只8周龄、雄性、平均体重为(220±15)g的Wistar大鼠随机分为COPD组(A组)、COPD+bFGF组(B组)、COPD+anti-bF-GF组(C组)和对照组(D组),每组10只。A、B、C3组COPD模型制备:经气管内注入脂多糖(LPS)和反复烟,复制成。A组为COPD对照组;B组:每周1次经尾静脉内注入0·1μgbFGF;C组:每周1次经尾静脉内注入0·1μganti-bFGF;D组:舱外饲养4周。4周后对各组大鼠气道进行病理学评分;检测并比较各组支气管平滑肌指数(平滑肌厚度/气道内径)及胶原指数(胶原厚度/气道内径);用免疫组化法观察bFGF在支气管肺内表达。结果与D组相比,A组、B组、C组大鼠的小气道都存在不同程度病理改变,差异均有显著性意义。与A组比较,B组肺组织病理评分显著增高、平均肺泡面积显著扩大、支气管平滑肌指数和胶原指数显著增高、bFGF吸光值均显著增高,而C组肺组织病理评分显著降低、平均肺泡面积显著缩小、支气管平滑肌指数和胶原指数显著降低、bF-GF吸光值均显著减少。结论bFGF参与了大鼠COPD模型气道炎症和气道重塑过程,而anti-bFGF可以抑制这一过程。Objective To observe the expression and distribution of the basic fibroblast growth factor （bFGF）in lung through chronic obstructive pulmonary disease （COPD） rat models and to investigate the potential role of bFGF in the mechanism for airway remodeling in COPD. Methods Forty male,8 weeks old Wistar rats were divided into groups randomly：COPD group;bFGF interfere in COPD group;anti-bFGF interfere in COPD group, and control group. COPD models were established by intratracheal instillation of lipopolysacchairde（LPS） twice in a month and exposure to cigarette smoking daily and in order to contrast respectively instillate particular quantity bFGF and anti-bFGF into different COPD model group from caudal vein in every week. After these models became true, observe the pathologic alteration of small airways, arterioles and pulmonary alveolus by hematoxylin and eosin stain Van-Gieson ＋ elastic fibers stain. At the same time, the thickness of the smooth muscle and collagen in bronchi and pulmonary arterioles were measured by computer image analyzer;also the protein and gene relative content of bFGF as well as the effects of antibody on them were observed by Histostaining and ISH （ in situ hybridization ） detection. Results There was a significant increase in the thickness of the smooth muscle and collagen in bronchi of bFGF interfere in COPD group compared with that of control group and COPD group （P 〈 0. 05 ） ;there was great decrease in anti-bFGF interfere in COPD group compared with that of control group and COPD group. The expression of the bFGF was greatly increased in tunica mucosa bronchiorum epithelium, pulmonary arteriole and lung alveolus macrophage（P 〈0. 01 ）. The relative content for bFGF interfere in COPD group was significantly higher than that of COPD group（P 〈 0. 01 ）. The alternation of anti-bFGF interfere in COPD group was greatly lower than that of COPD group. Conclusion bFGF might participate in the process of airway remodeling in COPD;anti-bFGF might restrain the

关 键 词：肺疾病 慢性阻塞性 碱性成纤维细胞生长因子 气道重塑 动物模型 

分 类 号：R5[医药卫生—内科学]