大田软海绵酸单克隆抗体ELISA检测方法的建立  

Establishment of McAb-based competitive ELISA for detection of okadaic acid

作　　者：卢士英[1] 周玉[1] 李岩松[1] 任洪林[1] 霍方珍[1] 王颖[1] 柳增善[1] 于光 

机构地区：[1]吉林大学教育部人兽共患病重点实验室,吉林长春130062 [2]吉林进出口检验检疫局,吉林长春130062

出　　处：《中国兽医学报》2007年第3期336-339,411,共5页Chinese Journal of Veterinary Science

基　　金：国家自然基金资助项目(30671762);国家质检总局资助项目(2003IK044)

摘　　要：利用BALB／c小鼠腹水大量制备抗大田软海绵酸（okadaic acid，OA）的单克隆抗体，并以此抗体为探针建立了0A的快速、灵敏、简便的ELISA检测方法——直接和间接竞争抑制性ELISA方法，2种检测方法的回归方程和相关系数分别为：y=-38．678χ＋130．01，R^2=0．9886和y=-34．212x＋83．49，R^2=0．9784，线性范围为1.56～75μg／L和0.4425μg／L，对OA的最低检出质量浓度为0．6μg／L和0．18μg／L。并利用所建立的2种ELISA方法对贝类模拟样品及实际样品进行了检测，样品回收率分别为84．72％和98．38％。结果表明，此方法可满足海产品中贝类样品0A限量标准检测。The ascitic fluids of anti-Okadaic Acid McAb was large-scale prepared by intraperitoneal injection in BALB/c mice and a fast, sensitive, convenient enzyme linked immunosorbent assay（ELISA） was set up. One was direct competitive inhibition ELISA, the other was indirect competitive inhibition ELISA; The standard curve for OA showed good linearity over the concentration range of 1.56-75μg/L and 0.4-25μg/L. The equation of linear regression werey = - 38.678x ＋ 130.01 and y =-34.212x ＋ 83.49, The coefficient correlation of the two methods wereR^2=0.978 4 and R^2=0.9886; The concentration limit to OA is 0.6μg/L and 0.18μg/L, The average recovery of sample being added OA was 84.72% and 98.38%. This metheod can be used for detecting the limit of OA in shellfish.

关 键 词：大田软海绵酸 单克隆抗体 ELISA 

分 类 号：S852.4[农业科学—基础兽医学] X503.225[农业科学—兽医学]