公牛精子分离前的保存  

Storage of bull spermatozoa prior to flow cytometric sorting

作　　者：陆阳清[1] 王武陵[2] 张明[1] 韦英明[1] 卢克焕[1] 

机构地区：[1]广西大学动物繁殖研究所 [2]吉首大学生物资源与环境科学学院,湖南吉首416000

出　　处：《中国兽医学报》2007年第3期420-422,共3页Chinese Journal of Veterinary Science

基　　金：国家"十五"重大科技专项资助项目(2002BA518A01);广西壮族自治区科技攻关资助项目(桂科攻0235016);广西壮族自治区教育厅科研资助项目(桂教科研[2002]136)

摘　　要：试验采用荷斯坦公牛精液,比较了添加抗生素、TALP稀释以及TALP稀释染色3种处理方法对常温下保存精子活力的影响。研究结果表明,新鲜精液加抗生素处理后直接保存的效果优于另外2个处理组,而分离精子经冷冻解冻后的存活率达40%,说明了该方法的可靠性。Raw semen from Holstein bull was stored respectively under the treatment of antibiotics, TALP dilution, TALP dilution plus fluorochrome staining. The results showed that the sperm motility in the treatment of antibiotics was the best, and the practice of such treatment in the transport and sorting of bull spermatozoa showed that the frozen-thawed motility was about 40%, indicative of a reliable procedure for the storage of spermatozoa prior to flow cytometric sorting.

关 键 词：公牛 精子 保存 流式细胞仪分离 

分 类 号：Q954.4[生物学—动物学] S814[农业科学—畜牧学]