中国水稻二化螟5个地理种群遗传差异的RAPD分析  

Genetic diversity among five geographical populations of Chilo suppressalis based on RAPD

作　　者：薛进[1] 苏建伟[2] 黎家文[1] 陈秋芳[1] 

机构地区：[1]湖南农业大学生物安全科学技术学院,湖南长沙410128 [2]中国科学院动物研究所农业虫鼠害综合治理研究国家重点实验室,北京100080

出　　处：《湖南农业大学学报（自然科学版）》2007年第2期160-163,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：中国科学院知识创新工程前沿项目(KSCX-IOZ-04);湖南农业大学青年科学基金项目(06QN09)

摘　　要：以中国水稻二化螟5个地理种群的基因组DNA为材料,对其进行随机扩增多态性DNA(RAPD)分析.从50个引物中筛选出29个稳定性好、多态性高的引物,单个引物扩增出的DNA条带数从2～15条不等,其片段大小为200～3 000 bp,5个地理种群共扩增出条带220条,其中为多态片段的97条,平均每条引物扩增出条带7.59条.通过公式计算多态性位点、遗传相似性指数和遗传距离,结果表明:(1)5个地理种群遗传变异较高,其中以广西省全州市多态位点百分率最高(52.73%),吉林省柳河县多态位点百分率最低(34.55%);(2)5个二化螟地理种群间的遗传相似性指数的变异范围为0.551 88～0.849 00,遗传距离指数为0.121 42～0.448 12,平均遗传距离指数为0.312 28;安徽省庐江县与湖北省武汉市地理种群间的遗传距离最小,而吉林省柳河县与广西省全州市地理种群间的遗传距离最大.Five geographical populations of Chilo suppressalis are detected by RAPD. Twenty-nine primers are screened from 50 random primers and amplified 220 bands（from 200 bp to 3 000 bp）. The number of obtaining segments of individual primer is between 2-15 with an average 7.59. 97 of these bands are polymorphic bands. Polymorphisms, genetic similarity indices and genetic distance were calculated. The results of the study are as below： （1） The genetic diversity among five geographical populations could be distinguished from the polymorphisms. Polymorphisms from Quanzhou, Guanxi are the smallest while that from Liuhe, Jilin are the biggest; （2） The genetic comparability of five geographical populations varies 0.551 88 to 0.849 00 and the range of genetic distance is from 0.121 42 to 0.448 12 and the average was 0.223 06. The genetic distance between the two populations from Lujiang, Anhui and Wuhan, Hubei is the smallest among five populations. However, the genetic distance between the two populations from Liuhe, Jilin and Quanzhou, Guangxi is the biggest.

关 键 词：二化螟 地理种群 遗传差异 随机扩增多态性DNA 

分 类 号：S435.112.1[农业科学—农业昆虫与害虫防治]