产R-肾上腺素脱氢酶菌种筛选与产酶条件研究  

Screening of Strain S289 with the Ability of Biosynthesis of R-adrenalin and the Study of R-adrenalin Dehydrogenase

作　　者：杨凯[1] 郭黛苹[1] 李忠琴[1] 陈倍[1] 陈必钦[1] 许小平[1] 

机构地区：[1]福州大学化学化工学院,福建福州350002

出　　处：《福建师范大学学报（自然科学版）》2007年第3期67-70,共4页Journal of Fujian Normal University：Natural Science Edition

基　　金：福建省自然科学基金(C0410006)

摘　　要：以肾上腺酮为底物,在1 300余份土壤中筛选到一株不对称还原肾上腺酮生成R-肾上腺素的菌株S289,产物得率为23.3%,对映体过量值e.e.高达99%,并对其产酶条件进行了研究,使其酶活和比酶活分别提高了168%和230%.A new strain S289 was isolated from soil using corticosterone as the substrate, which can biosynthesize R-adrenalin by asymmetric bioreduction of corticosterone, the yield and the enantiomeric excess of R-adrenalin were 23.3% and 99%. After study of the conditions of dehydrogenase production, the total activity and specific activity were enhanced 168% and 230% respectively.

关 键 词：筛选 R-肾上腺素脱氢酶 生物转化 

分 类 号：Q554[生物学—生物化学]