磁靶纳米化疗药物治疗胆管癌移植瘤的对比研究  

A comparative study of targeting drug magnetic nanoparticles and other chemotherapy drugs on human cholangiocarcinoma xenografts

作　　者：唐滔[1] 郑建伟[1] 李宏[1] 肖宝来[1] 陈波[1] 李高[2] 许帅[3] 邹声泉[1] 艾星[4] 李曦[5] 

机构地区：[1]华中科技大学同济医学院附属同济医院普通外科,湖北武汉430030 [2]华中科技大学同济医学院附属同济医院药学院,湖北武汉430030 [3]武汉理工大学材料复合新技术国家重点实验室,湖北武汉430070 [4]华中科技大学同济医学院附属同济医院泌尿外科,湖北武汉430030 [5]武汉大学人民医院消化内科,湖北武汉430060

出　　处：《中国普通外科杂志》2007年第2期121-124,共4页China Journal of General Surgery

基　　金：国家863高技术研究发展计划资助项目(2002AA214061)。

摘　　要：目的比较纳米磁靶向性药囊与其他化疗药物治疗裸鼠人胆管癌移植瘤的效果并探讨其机制。方法以在体外培养的人胆管癌细胞QBC939接种裸鼠。20d后将裸鼠随机分为4组(每组8只)生理盐水对照组(A组),5-FU治疗组(B组),纳米磁靶向性药囊+内磁化支架治疗组(C组),健择治疗组(D组)。记录各组各时段肿瘤体积变化和抑瘤率,绘制肿瘤生长曲线。于治疗后35d处死裸鼠,取肿瘤组织观察超微结构改变。结果A,B,C,D组于治疗后35d肿瘤体积分别为(2256.1±267.1)mm3,(2096.5±237.9)mm3,(1392.2±189)mm3,(1534.9±115)mm3,其中C,D组与A,B组之间的肿瘤体积比较有明显差异(P<0.05)。B,C,D组抑瘤率为7.4%,39.6%和33%。电境观察C组和D组均有凋亡细胞出现。结论同等剂量下,纳米磁靶向性药囊治疗效果优于5-FU,但与健择无明显差异。Objective To study the effect of magnetic nanoparticles on human cholangioearcinoma xenograft in nude mice, and compared with other chemotherapy drugs Methods We established human cholangioearcinoma xenograft in nude mice with QBC939 cell line. The nude mice were devided into 4 groups randomly. Saline, 5-FU, Gemcitabine and magnetic nanoparticles were given to nude mice through tail vein on 20d after implanting QBC939 cell line. Calculations were done at different time after treatment in order to compare tumor volume, inhibition ratio of tumor and tumor growth curve of each group. The nude mice were killed on 35 d after treatment to harvest tissue for electron microscopic examination to observe ultra-structural changes. Results The tumor volume of control, 5-FU, magnetic nanoparticles and Gemcitabine groups was （ 2256. 1 ± 267. 1 ） mm3 , （ 2096.5 ± 237.9 ） mm3 , （ 1392.2 ± 189） mm3 , and （ 1534. 9 ± 115 ） mm3 respectively. The last two groups have significant difference compared to the first two groups （ P 〈 0.05 ）. The ratio of inhibition of tumor and apotosis in the chemotherapy treated groups were 7.4 % , 39.6 % , and 33.0 % respectively. Conclusions The effect of targeting drug magnetic nanoparticles was better than equivalent dosage of 5-FU, but as the same of Gemcitabine .

关 键 词：胆管肿瘤/药物疗法 肿瘤 实验性 肿瘤移植 磁靶纳米化疗药物/治疗应用 

分 类 号：R657.4[医药卫生—外科学]