建立小鼠腹部心脏移植模型的体会  

Ventral heterotopic heart transplantation in mice

作　　者：朱鹏[1] 陈义发[1] 张宜江[1] 陈孝平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肝脏外科中心,湖北武汉430030

出　　处：《中国普通外科杂志》2007年第2期133-135,共3页China Journal of General Surgery

基　　金：国家自然基金资助项目(30571754)。

摘　　要：目的探讨小鼠腹部异位心脏移植手术的技术方法及其改进,为进行移植免疫学研究提供动物模型。方法采用供心主动脉与受体腹主动脉、供心肺动脉与受体下腔静脉端侧吻合方法对100例小鼠进行腹部心脏移植术,同时测量相关移植部位的重要解剖结构。结果供心主动脉直径为(1.0±0.1)mm,供心肺动脉直径为(1.3±0.2)mm;受体下腔静脉直径为(1.9±0.2)mm,腹主动脉直径为(0.9±0.1)mm,腹腔大血管平均可利用长度为(6.0±1.0)mm。建模成功率为92%。供、受体手术时间分别为(8.0±1.0)min和(40.2±3.0)min;其中动脉吻合为(9.3±1.2)min,静脉吻合为(7.8±1.7)min。结论所建立的小鼠心脏移植模型稳定可靠,适用于移植免疫学方面的研究。Objective To explore an improved technique of mouse ventral heterotopic heart transplantation and construct an animal model for the study of transplantation immunity. Methods The donor heart aorta and the recipient ventral aorta, the donor pulmonary artery and the recipient inferior caval vein, were anastomosed by using the end-to-side suture technique respectively. At the same time, important anatomical structures of transplantation-related sites were measured. Results The diameter of recipient inferior caval vein and ventral aorta was （ 1. 9± 0. 2 ） mm and （0.9±0.1）mm respectively. The average length of ventral blood vessels which can be used was （6.0±1.0）mm. The model construction success rate was 92%. The average time of donor operation and recipient operation was （8.0±1.0）min and （40.2 ±3.0）min respectively. The time of artery anastomosis and vena cava anastomosis was （9.3 ±1.2）min and （7. 8 ± 1. 7 ）min respectively. Conclusions The animal model is stable and can be used for the study of transplantation immunity.

关 键 词：心脏移植/方法 模型 动物 腹部 

分 类 号：R617[医药卫生—外科学]