人用禽流感疫苗H5N1株三级毒种库的建立  

Construction of human avian influenza H5N1 vaccine seed library

作　　者：张松乐[1] 邢丽[1] 罗德炎[1] 何蕊[1] 杨海荣[1] 张钰[1] 王希良[1] 

机构地区：[1]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071

出　　处：《军事医学科学院院刊》2007年第2期107-109,113,共4页Bulletin of the Academy of Military Medical Sciences

基　　金：国家防控禽流感科技攻关项目(2004BA519A70)

摘　　要：目的：建立人用禽流感疫苗H5N1株三级毒种库，为生产疫苗提供毒种。方法：用SPF鸡胚培养病毒，对型别、滴度和无菌等各项检测合格后，建立人用禽流感疫苗H5N1株三级毒种库。结果：在33℃培养52h条件下，毒种滴度为1：640-1：1280；厌氧菌、细菌、真菌、支原体、血吸附病毒、非血吸附病毒和外源性禽白血病病毒检测均为阴性。将毒种在鸡胚上传至12代，滴度在1：320-1：640之间，中和抗体滴度在1：160以上，EID50在6．7以上；毒种HA、NA基因序列检测与公布的HA、NA基因序列的同源性达99．0％，代次之间达99．9％。结论：本工作为研制人用禽流感疫苗贮备了标准毒种。Objective： To provide virus seeds for vaccine production by constructing the third level seed library of human avian influenza H5N1 virus. Methods：Influenza virus was cultivated in SPF chick embryo and the type,titer, sterility etc. were identified. Results：The HA titers were 1：640 - 1：1 280 at 33℃ ,52 hours. Anaerobian, bacteria, fungi, mycoplasma and exogenous avian leukemia virus and hemadsorption and non-hernadsorption viruses were negative. The HA titers, EID50 of the virus and HI titers of immunized mice 28 days after injection were 1： 320 - 1： 640, over 6.7 and above 1： 160, respectively of the 12 passages. The autoploidy of HA, NA sequences with reference sequences were 99.0% and that between the passages were 99.9%. Conclusion：The seed library may be used as the seeds for developing human avian influenza H5N1 vaccine.

关 键 词：流感 禽 鸡胚 流感疫苗 H5N1 毒种库 

分 类 号：R392.1[医药卫生—免疫学]