人参与藜芦配伍化学成分变化的HPLC-ESI-MS与ESI-MS研究  

Studies on the Content Variation of Chemical Constituents During the Combination of Ginseng with Veratrum nigrum by ESI-MS and HPLC-ESI-MS

作　　者：张旭[1] 宋凤瑞[1] 王隶书[2] 刘志强[1] 刘淑莹[1] 

机构地区：[1]中国科学院长春应用化学研究所长春质谱中心 [2]长春中医药大学,长春130117

出　　处：《化学学报》2007年第9期829-833,共5页Acta Chimica Sinica

基　　金：国家自然科学基金(No.30472134);中国科学院知识创新工程重要方向(No.KGCX2-SW-213-06)资助项目.

摘　　要：采用电喷雾质谱(ESI-MS)和高效液相色谱与电喷雾质谱联用技术(HPLC-ESI-MS),对人参与藜芦配伍过程中人参皂苷和藜芦生物碱的变化规律进行了系统研究.在人参与藜芦配伍的共煎液中鉴定出八种人参皂苷,其中有六种人参皂苷含量有所降低,Rf和Rb2的含量基本不变;此外还鉴定出八种藜芦生物碱,其中有六种生物碱的含量随人参加入而明显增高.人参与藜芦配伍,煎煮液中人参皂苷的含量下降,藜芦总碱的含量上升.人参的加入有利于藜芦生物碱的溶出.因藜芦总碱的毒性较强,所以人参“反”藜芦具有一定道理.To study the content variation of ginsenosides and alkaloids during combination of ginseng with veratrum nigrum, the ginsenosides and alkaloids in the decoction of ginseng with veratrum nigrum were analyzed and compared by high performance liquid chromatography-electrospray ionization-mass spectrometry （HPLC-ESI-MS） and electrospray ionization-mass spectrometry （ESI-MS）. In the compatible decoction, eight ginsenosides and eight alkaloids were detected, and the contents of six ginsenosides were found to be reduced, on the contrary, the contents of six alkaloids were increased. During combination of ginseng with veratrum nigrum, the contents of ginsenosides were reduced and those of the toxic alkaloids were increased. From the chemical point of view, the traditional theory is right that ginseng and veratrum nigrum are incompatible with each other.

关 键 词：人参 人参皂苷 藜芦 生物碱 配伍 质谱 液质联用 

分 类 号：R284[医药卫生—中药学]