与南瓜矮生基因连锁的分子标记  

Molecular Markers Linked to the Dwarf Gene in Squash

作　　者：李云龙[1] 李海真[1] 崔崇士[2] 张海英[1] 宫国义[1] 

机构地区：[1]国家蔬菜工程技术研究中心,北京100089 [2]东北农业大学园艺系,哈尔滨150030

出　　处：《农业生物技术学报》2007年第2期279-282,共4页Journal of Agricultural Biotechnology

基　　金：北京市自然科学基金(No.5042008);北京市优秀人才培养专项经费项目;山西省自然科学基金项目(No.20011096)资助。

摘　　要：以中国南瓜（Cucurbita moschata Duch）矮生突变体（矮10）为供体亲本，以印度蔓生南瓜（Cmax／maOuch）（蒙El南瓜）为轮回亲本，经6代回交和2代自交选育出s1～s9共9个南瓜矮生近等基因纯合株系，其中s2株系再与蒙日南瓜杂交获得F2分离群体共306株。利用RAPD技术，采用近等基因系（nearisogenic lines，NILs）分析法，结果表明，在640条随机引物中，发现RAPD标记S1225-548与矮生单基因D呈现连锁关系，遗传距离为2．29cM。RAPD标记成功转化成更为稳定的SCAR标记SCAR3-398。The first discovered dwarf mutant （Ai 10）of the Cucurbita moschata Duch was used as the donor parent, and the normal C. maxima Duch （Mengri） was used as recurrent parent. Nine pure lines of the near isogenic lines （NILs） named S1~$9 were got through the 6 times backcrosses and twice selfcrosses. The 306 F2 individuals were derived from the single cross between the NIL $2 and Mengri. All of the 640 random primers were screened for the marker linked to the dwarf gene D using RAPD and NILs approaches. The results showed that the RAPD marker S1225-548 was linked to the dwarf gene. The genetic distance was 2.29 cM, and it has been transformed to SCAR marker SCAR3-398.

关 键 词：南瓜 近等基因系 矮生基因D RAPD SCAR 

分 类 号：S184[农业科学—农业基础科学] S188