中国部分板栗品种的SSR标记  

Development and Characterization of SSR Markers in Chinese Patial Chestnut(Castanea mollissima) Cultivars

作　　者：艾呈祥[1] 余贤美[2] 张力思[1] 刘庆忠[1] 

机构地区：[1]山东省果树研究所,山东省果树生物技术育种重点实验室,泰安271000 [2]中国热带农业科学院环境与植物保护研究所,儋州571737

出　　处：《农业生物技术学报》2007年第2期283-289,共7页Journal of Agricultural Biotechnology

基　　金：国家科技基础性工作项目(No.2004DKA30390-20);山东省农业科学院青年基金项目(No.2005YQ013)资助。

摘　　要：从板栗（Castanea mollissima）燕红中分离到25个SSR标记。为了鉴定这些SSR位点，在重复序列的两侧侧翼序列设计引物，并通过化学荧光检测法对6个板栗样品进行检测，共检测到20个多态性位点，每个位点的等位基因数为2-6个。挑选12个位点，通过半自动系统ABIPRISM377对中国北方24个板栗品种进行分析，这12个标记显示了高达75个等位基因的遗传多态性，每个位点的等位基因为4-10个，平均为6-3个，预期的平均杂合性为0．743（介于0．680-0．845），观察值为0．829（介于0．7304）．930）。无效等位基因估算频率显示为3个位点的正向价值，除了一个位点外，这些价值都很低，鉴定几率为7．01×10^-11，亲缘关系鉴定几率非常高，为0．999，足够用于花粉流的研究。Twenty-five simple sequence repeat （SSR） markers were isolated and characterized in chestnut （Castanea mollissim ） from the cultivar Yanhong. For the identification of SSR locl, primers were designed on each side flanking the repeat region and they were initially tested on 6 chestnut samples using chemiluminesence detection. Twenty loci were shown to be polymorphic and the number of alleles detected per locus varied from 2to 6, Twelve 10ci were chosen for the analysis of 24 cultivars grown in North Chinese using the semi-automatic system ABI PRISM 377. The 12 markers showed a high level of genetic polymorphism with a total of 75 alleles; the number of alleles ranged, from 4 to 10 per locus, with an average level of 6.3. The mean expected and observed heterozygosity were 0.743 （range from 0.680 to 0.845） and 0.829 （range from 0.730 to 0.930）, respectively. The estimated frequence of null alleles showed a positive value for 3 loci, but the values were very low except for 1 locus. The total value for the probability of identity was 7.01×10^-11. Paternity exclusion probability was 0.999, and was sufficiently high to study pollen flow.

关 键 词：板栗 二核苷酸 DNA分型 多态性 SSR标记 

分 类 号：S188[农业科学—农业基础科学]