Toll样受体在慢性鼻-鼻窦炎和鼻息肉中表达的差异  

Expression difference of Toll-like receptors among chronic rhinosinusitis and nasal polyps

作　　者：游学俊[1] 陆翔 刘洋[2] 刘争[1] 高起学[1] 崔永华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院耳鼻咽喉头颈外科,武汉430030 [2]北京中日友好医院耳鼻咽喉头颈外科

出　　处：《中华耳鼻咽喉头颈外科杂志》2007年第5期345-349,共5页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：国家自然科学基金(30500557);2003年湖北省卫生厅科研基金(JX1B046)

摘　　要：目的检测 Toll 样受体-2(Toll-like receptor-2,TLR-2)和 Toll 样受体4(Toll-likereceptor-4,TLR-4)mRNA 及蛋白在慢性鼻-鼻窦炎、鼻息肉和正常下鼻甲鼻黏膜组织中的表达及表达程度的差异,探讨其在慢性鼻-鼻窦炎和鼻息肉发病机制中的作用。方法采用反转录聚合酶链式反应(reverse transcriptase-polymerse chain reaction,RT-PCR)分别检测10例慢性鼻-鼻窦炎筛窦黏膜、10例鼻息肉和10例下鼻甲黏膜组织中 TLR-2和 TLR-4 mRNA 的表达;免疫组织化学技术检测20例慢性鼻-鼻窦炎筛窦黏膜、20例鼻息肉和20例下鼻甲黏膜组织中 TLR-2和 TLR-4蛋白的表达和分布,并比较表达程度的差异。结果①TLR-2、TLR-4 mRNA 及蛋白在慢性鼻-鼻窦炎、鼻息肉和下鼻甲组织中均有表达,免疫组化显示蛋白主要表达在鼻黏膜上皮细胞及腺体细胞朝向鼻腔或腺体管腔一侧的细胞膜;②慢性鼻-鼻窦炎组织中 TLR-2 mRNA 及蛋白和 TLR-4 mRNA 表达水平高于鼻息肉和下鼻甲组织,差异有统计学意义(P 值均<0.05)。③鼻息肉组织中 TLR-2、TLR4 mRNA 及蛋白表达水平与下鼻甲组织相比差异无统计学意义(P 值均>0.05)。结论 TLR-2和 TLR-4在慢性鼻-鼻窦炎、鼻息肉和正常下鼻甲黏膜上皮细胞膜上均有表达;慢性鼻-鼻窦炎和鼻息肉组织中 TLR-2、TLR-4 mRNA及蛋白的表达量不同,提示 TLR-2和 TLR-4在两者的发病机制中起不同的作用。Objective To compare the expression difference of Toll-like receptor-2 （TLR-2） and Toll-like receptor-4 （TLR-4） protein and mRNA among the chronic rhinosinusitis tissues, nasal polyps tissues,and normal mucosa tissues. Methods The mRNA expression of TLR-2 and TLR-4 in chronic rhinosinusitis tissues from 10 patients, in nasal polyp tissues from 10 patients, and in inferior turbinate tissues from 10 patients underwent nasal septum operation was detected by means of reverse transcriptasepolymerse chain reaction （RT-PCR）. Immunohisto-chemistry （IHC） was used to detected the expression of TLR-2 and TLR-4 protein in a different set of 20 chronic rhinosinusitis tissues, 20 nasal polyp tissues, and 20 normal inferior turbinate tissues. Results ①The mRNA and protein expression of TLR-2 and TLR-4 was detected on epithelial and glandular cells membrane in all chronic rhinosinusitis, nasal polyps and control tissues. ② The mRNA and protein expression of TLR-2 and the mRNA expression of TLR-4 in chronic rhinosinusitis tissues was significantly increased compared with that in nasal polyps and control tissues （P 〈 0. 05）. ③ The expression intensity of TLR-2 and TLR-4 mRNA and protein between nasal polyps and control tissues was found no significant difference （P 〉 0. 05 ）. Conclusions Different TLR-2 and TLR-4 protein and mRNA level in chronic rhinosinusitis and nasal polyp tissues might imply that TLR-2 and TLR-4 play different role in the pathogenesis of chronic rhinosinusitis and nasal polyps.

关 键 词：鼻窦炎 鼻息肉 Toll样受体-2 TOLL样受体-4 

分 类 号：R765[医药卫生—耳鼻咽喉科]