H^+参与茉莉酸调控蚕豆气孔运动的信号转导  

H^+ Involving Signal Transduction in Regulation of Vicia faba L. Stomatal Movement by Jasmonic Acid

作　　者：刘新[1] 李云[1] 孟繁霞[1] 张蜀秋[1] 

机构地区：[1]中国农业大学生物学院植物生理学与生物化学国家重点实验室

出　　处：《植物生理学通讯》2007年第2期245-249,共5页Plant Physiology Communications

基　　金：国家自然科学基金(30370141);山东省教育厅基金(J04C13)。

摘　　要：以BCECF-AM为pH的荧光探针,结合激光共聚焦扫描显微技术,研究H+可能参与茉莉酸(JA)调控气孔运动信号转导途径的结果表明,0.1~100μmol·L^(-1)浓度的(-)JA可诱导蚕豆气孔关闭,在引起气孔孔径改变之前,(-)JA能引起蚕豆保卫细胞胞质的碱化;而(±)JA可诱导气孔适当开放,它未引起蚕豆保卫细胞胞质中pH的明显改变。药理学实验证明,质膜上质子泵的抑制剂矾酸钠能减弱(-)JA诱导气孔关闭的作用;而质膜上质子泵的激活剂壳梭孢菌素(fusicoccin)基本上未改变(±)JA的作用趋势。(-)JA和(±)JA刺激保卫细胞胞质Ca2+变化则表现出不同趋势。说明不同异构体形式的JA在调节气孔运动中的作用和信号转导途径有所不同。Using fluorescent probe BCECF-AM and Fluo-3 monitored the changes of pHi and [Ca^2＋]cyt in guard cells of Viciafaba under confocal microscopy. Whether H^＋ was involved in signal transduction in JA regulating stomatal movement was tested. The results showed that （-）JA induced stomatal closure at concentrations of 0.1-100 μmol·L^-1 was mediated intracellular alkalization in guard cells. In contrast, （＋）JA induced stomatal open without pHi changes. Pharmacological experiment proved that Na3VO4 reduced the effectiveness of （-）JA-induced stomatal closure, while fusicoccin had no effect on （＋）JA-induced stomatal opening. Compared changes of [Ca^2＋]cyt induced by （-）JA and （＋）JA： （-）JA increased [Ca^2＋]cyt rapidly, while （＋）JA induced [Ca^2＋]cyt increasing and decreasing slowly. These results demonstrated that （-）JA and （＋）JA had different roles and signal pathways in regulation of stomatal movement.

关 键 词：茉莉酸 气孔运动 H^+ CA^2+ 信号转导 蚕豆 

分 类 号：Q945.1[生物学—植物学]