拟南芥干旱诱导型启动子RD29A驱动花生AhNCED1基因双元表达载体的构建  

Construction of Binary Vector Harboring Peanut AhNCED1 Gene Driven by Drought-inducible Promoter of RD29A Gene from Arabidopsis

作　　者：万小荣[1] 李玲[2] 

机构地区：[1]仲恺农业技术学院生命科学学院,广州510225 [2]华南师范大学生命科学学院,广州510631

出　　处：《植物生理学通讯》2007年第2期283-287,共5页Plant Physiology Communications

基　　金：广东省自然科学基金(06301202);仲恺农业技术学院博士启动基金(G2360225);广东省自然科学基金(06025049)。

摘　　要：从拟南芥基因组中克隆RD29A基因5'-侧翼520bp启动子区域序列,生物信息学分析表明,该启动子片段中存在脱水胁迫响应元件(DRE)、ABA响应元件(ABRE)、TATA-box、CAAT-box等顺式作用元件。构建了干旱诱导型启动子AtRD29Ap驱动花生AhNCED1基因的植物双元表达载体pAtRD29Ap::AhNCED1。In this paper we report the cloning of 5＇-flanking promoter sequence of drought-inducible RD29A gene from Arabidopsis and its fusion with peanut AhNCED1 gene. The 520-bp promoter sequence was ana- lyzed bioinformatically in the database of plant cis-acting regulatory element （PlantCARE）. The result showed that there were several important cis-acting elements, including TATA-box, CAAT-box, DRE （response to dehydration）, ABRE （response to ABA）, in the 520-bp promoter region. The binary vector harboring AhNCED1 gene driven by AtRD29A promoter was further constructed to be used in coming plant transgene.

关 键 词：RD29A基因启动子 NCED基因 双元载体构建 

分 类 号：Q943.2[生物学—植物学]