年轻与老龄小鼠未成熟卵母细胞之间的生发泡移植  

Germinal Vesicle Transfer between Young and Aged Mouse Immature Oocytes

作　　者：崔龙波[1] 周雪莹[1] 黄秀英[2] 孙方臻[2] 

机构地区：[1]烟台大学生物化学系,烟台264005 [2]中国科学院遗传与发育生物学研究所,北京100080

出　　处：《细胞生物学杂志》2007年第2期232-236,共5页Chinese Journal of Cell Biology

基　　金：国家重点基础研究发展规划项目(973计划)(No.G1999055902)资助~~

摘　　要：为研究不同年龄来源的细胞质或细胞核对卵母细胞成熟、钙振荡及核型的影响，在6罐周龄（6w）小鼠与9月龄（9M）和12月龄（12M）小鼠卵母细胞之间进行了生发泡（GV）互换。通过显微操作和电融合获得了5组重组卵母细胞。重组卵母细胞和对照卵母细胞经Sr^2＋诱导后呈现相似的钙震荡模式。6WGV-6W胞质体组、6WGV-9M胞质体组和6WGV-12M胞质体组成熟卵母细胞染色单体提早分离的比率与6～8周龄对照组比较无差异（P〉0.05），但显著低于12月龄对照组（P〈0．01）。而9MGV-6W胞质体组和12MGV-6W胞质体组成熟卵母细胞染色单体提早分离的比率则明显增加。这些结果表明由年轻与老龄小鼠之间GV互换所重组的卵母细胞能够正常成熟和产生钙震荡，与衰老相关的减数分裂异常似乎归因于细胞核或染色体而不是细胞质。To elucidate effects of nucleus or cytoplasm from different age of animals on oocyte maturation, Ca^2＋ oscillations and karyotypic status, germinal vesicles were exchanged between immature oocytes from mice of 6-8 weeks （6W）, 9 months （9M） and 12 months （12M）. Five groups of reconstructed oocytes were obtained by micromanipulation and electrofusion. Oocytes in five experiment groups and three control groups showed a similar pattern of Ca^2＋ oscillations by Sr^2＋ induction. The rates of the matured oocytes with premature chromatid separation for group 6W GV-6W cytoplast, group 6W GV-9M cytoplast and group 6W GV-12M cytoplast were comparable to those of control 6-8 weeks （P〉0.05）, but significantly lower than those of control 12 months （P〈0.01）. However, the rates of the matured oocytes with premature chromatid separation increased for group 9M GV-6W cytoplast and group 12M GV-6W cytoplast. These findings demonstrate that oocytes reconstructed by GV exchange between young and aged mice show normal maturation and Ca^2＋ oscillation. It seems that the aged-related meiotic anomalies may be due to nucleus or chromosomes rather than cytoplasm.

关 键 词：年龄 卵母细胞 生发泡移植 钙震荡 染色体 

分 类 号：Q813[生物学—生物工程]