5-HT_(1A)受体对双相呼气和吸气神经元电活动的调制(英文)  

5-HT_(1A) receptors are involved in the modulation of discharge activities of biphasic expiratory neurons and inspiratory neurons

作　　者：秦峥[1] 王晓锋[2] 郭谦[3] 吴中海[1] 

机构地区：[1]南方医科大学生理学教研室,广州510515 [2]南方医科大学南方医院神经内科,广州510515 [3]广州军区联勤部门诊部,广州510080

出　　处：《中华神经医学杂志》2007年第5期446-450,共5页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金(30570670);广东省自然科学基金(5004714)

摘　　要：目的探讨5-HT_(1A)受体对延髓脑片双相呼气神经元和吸气神经元电活动的影响。方法在新生大鼠延髓脑片上同步记录舌下神经根和双相呼气神经元/吸气神经元单位的放电活动,并在灌流的改良Kreb's液中先后加以5-HT_(1A)受体的特异性激动剂(+/-)-8-hydroxy-2- (di-N-propylamino)tetralin hydrobromide(8-OH-DPAT)和特异性拮抗剂多次甲基多苯基多异氰酸酯[4-iodo-N-[2-[4一(methoxyphenyl)-1-piperazinyl]ethyl]-N-2-pyridynyl-benzamide hydrochloride](PMPPI)观察对神经元单位放电的影响。结果给予5-HT_(1A)受体激动剂8-OH-DPAT后,双相呼气神经元/吸气神经元的呼吸周期和呼气时程明显延长,积分幅度降低,单位放电峰频率显著性降低;给予特异性拮抗剂PMPPI后,对呼吸周期,呼气时程的作用相反,而积分幅度和单位放电峰频率无明显变化。结论5-HT_(1A)受体可能通过影响双相呼气神经元的电活动参与了呼吸时相的转换,同时也可能介导了吸气神经元的抑制性突触输入。Objective To determine whether 5-HT1A receptors could modulate the discharge activities of biphasic expiratory neurons （BE-neurons） and inspiratory neurons （I-neurons）. Methods Brainstem slices from newborn SD rats （0-3 d） were made according to the method of Suzue, et al. These preparations included the medial region of the nucleus retrofacialis （mNRF） with the hypoglossal nerve rootlets retained. Respiratory rhythmical discharge activities （RRDA） of BE-neurons/I-neurons in mNRF and activities of the hypoglossal nerve （Ⅻ nerve） were simultaneously recorded by using extracellular microelectrodes and suction electrode, respectively. The effects of 5-HT1A receptors on the respiratory rhythm were investigated by application of 5-HT1A receptor specific agonist 8-OH-DPAT and its specific antagonist PMPPI in the perfusion solution. Results 8-OHDPAT induced a significant increase in the respiratory cycles （RC） and the expiratory time （TE） as well as a decrease in the integral amplitude （IA） and the peak discharge frequency （PFn） of RRDA of BE-neurons and I-neurons. By contrast, PMPPI produced opposite effects on the BE-neurons and I-neurons except that PMPPI changed IA and PFn of BE-neurons/I-neurons nonsignificantly. Conclusion 5-HT1A receptors are involved in the phase-switching between expiration and inspiration by affecting BE-neurons; 5-HT1A receptors are also possibly involved in the modulation of rhythmical respiration through the inhibitory synaptic input from I-neurons.

关 键 词：5-HT1A受体 双相呼气神经元 吸气神经元 呼吸节律 

分 类 号：R338.2[医药卫生—人体生理学]