表没食子儿茶素没食子酸酚对β-淀粉样多肽损伤的星形胶质细胞的保护作用  

Effect of tea polyphenol (-)-epigaUocatechin gallate on astrocyte injury induced byβ-amyloid peptide

作　　者：崔光彬[1] 杨浩[2] 魂经国 王玮[1] 魏龙晓[1] 王亚蓉[1] 宋立军[1] 李玮[1] 

机构地区：[1]第四军医大学唐都医院放射科,西安710038 [2]第四军医大学全军神经科学研究所,西安710038

出　　处：《中华神经医学杂志》2007年第5期464-467,477,共5页Chinese Journal of Neuromedicine

摘　　要：目的研究表没食子儿茶素没食子酸酚(EGCG)对异常蛋白β-淀粉样多肽(Aβ)损伤星形胶质细胞(AS)的保护作用,进一步探讨其作用机理。方法采用原代细胞培养技术,分离培养新生1 d大鼠皮质AS,细胞纯化后,加入Aβ进行处理,随后加入不同浓度(0.1、1、5、10、25、50μmol/L)的EGCG培养24h,观察细胞的形态学变化:MTT比色法进行细胞活性分析、AS中丙二醛(MDA)以及培养液中乳酸脱氧酶(LDH)含量的变化的检测;比较上述指标在损伤前后的变化。结果EGCG能够减轻Aβ对AS的损伤,抑制AS的MDA的产生,降低AS外液中LDH的含量,增强细胞的活性。结论EGCG能够抑制Aβ对AS的损伤作用,并且对其有神经保护作用.这种保护作用可能与其抑制Aβ引起细胞过度氰化有关。Objective To investigate the protective effect of tea polyphenol （-） -epigallocatechin gallate（EGCG）against astrocyte injury induced by amyloid β（Aβ） peptide and explore its protective mechanisms. Methods Primarily cultured astrocytes from postnatal 1 d rat were prepared and purified and subsequently treated with Aβ peptide to establish injury models. Then EGCG at different concentrations （0.1,1,5,10,25,50 umol/L） was also added into the cell culture at a concentration of 10 umol/L and treated these cells for 24 h. Morphological changes of the cells were then observed. Additionally, the astrocyte viability was determined by MTT assay and the content of malondialdehyde （MDA） in the cultured astrocytes and the level of lactate dehydrogenase （LDH） in the culture medium were detected and compared among different groups. Results EGCG reduced the Aβ-induced injury in the cultured astrocytes, inhibited the production of MDA in the cultured astrocytes, decreased the level of LDH in culture medium, and enhanced the astrocyte viability. Conclusion EGCG can attenuate the astrocyte injury caused by Aβ, and has neuroprotective effects on astrocytes maybe throught inhibiting cellular peroxidation induced by Aβ.

关 键 词：表没食子儿茶素没食子酸酚 星形胶质细胞 Β-淀粉样多肽 细胞培养 

分 类 号：R285.5[医药卫生—中药学]