金雀异黄素抑制单核细胞趋化蛋白-1诱导的人脐静脉内皮细胞凋亡  

Inhibitory effect of geinistein on apoptosis in hUVECs induced by MCP-1

作　　者：李琴山[1] 刘洋[1] 冯赞杰[1] 钱民章[1] 

机构地区：[1]贵州省遵义医学院生化教研室,贵州遵义563003

出　　处：《中国病理生理杂志》2007年第5期874-879,共6页Chinese Journal of Pathophysiology

基　　金：贵州省科学技术基金资助项目(No.2003-3015);遵义医学院硕士启动基金资助项目

摘　　要：目的:研究金雀异黄素对单核细胞趋化蛋白-1(MCP-1)诱导人脐静脉内皮细胞(hUVECs)凋亡的影响及可能机制。方法:培养并鉴定人脐静脉内皮细胞;用不同浓度金雀异黄素(0.1μmol、1μmol、10μmol、50μmol)和终浓度为10μg/L的MCP-1作用24h;先用MTT比色法观察细胞存活率、流式细胞技术检测细胞DNA含量及细胞周期,观察其对hUVECs生长的影响,流式细胞技术及Western印迹法检测凋亡相关蛋白Fas、Bcl-2、Bax的表达,探讨金雀异黄素对MCP-1诱导hUVECs凋亡干预的可能机制。结果:金雀异黄素抑制单核细胞趋化蛋白-1诱导的人脐静脉内皮细胞凋亡,抑制效应随剂量增加而增强(P<0.01,P<0.01,P<0.01)。MTT值明显增高;凋亡细胞明显少于对照组(10μg/LMCP-1),Bcl-2表达明显高于对照组,Fas、Bax表达明显低于对照组。结论:金雀异黄素能抑制单核细胞趋化蛋白-1诱导的人脐静脉内皮细胞的凋亡,抑制作用有量效相关性,抑制机制可能与下调Fas、Bax蛋白及上调Bcl-2蛋白的表达有关。AIM： To study the inhibitory effect of genistein on apoptosis in human umbilical vein endothelial cells （hUVECs） induced by monocyte chemotactic protein - 1 （ MCP - 1 ）. METHODS. The hUVECs were cultured in vitro and identified. Growth - arrested hUVECs were stimulated with genistein at different concentrations （0. 1 }μmol, 1.0 μmol, 10 μmol, 100 μmol） and co - treated with MCP - 1 （ 10μg/L）. The survival rates of hUVECs were detected by MTT assay. The cell cycle and DNA content were detected by flow cytometry. To explore the possible mechanism of the genistein interventions, the expressions of Bcl - 2, Fas and Bax proteins were detected by flow cytometry and Western blotting. RESULTS ： Genistein increased the survival rate and the level of Bcl - 2, inhibited Fas and Bax, decreased the ratios of apoptosis compared with MCP - 1 - induced hUVECs apoptotic group in a dose - dependent - manner. CONCLUSION： Genistein inhibits the apoptosis induced by MCP - 1 and the inhibitory effect was relative to the dose of genistein. Its mechanism might be involved in the down - regulation of Fas and Bax expressions and the up - regulation of Bcl - 2.

关 键 词：单核细胞化学吸引蛋白质1 染料木黄酮 脐静脉内皮细胞 蛋白质BAX 蛋白质Fas 蛋白质Bel-2 细胞凋亡 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]