γ粒子核素支架影响血管平滑肌细胞增殖与凋亡的实验研究  

Effect of γ radioactive stent on proliferation and apoptosis of smooth muscle cells

作　　者：刘英梅[1] 傅岳武[2] 孙立[3] 韦育林[1] 伍卫[1] 

机构地区：[1]中山大学附属第二医院心内科 [2]广东省中医院外科,广东广州510120 [3]暨南大学医学院,广东广州510632

出　　处：《中国病理生理杂志》2007年第5期884-887,共4页Chinese Journal of Pathophysiology

基　　金：卫生部科研基金资助项目(No.98-1-087)

摘　　要：目的:观察γ粒子钯-103[103Pd]核素支架对损伤血管中膜平滑肌细胞增殖与凋亡的影响。探讨核素支架防治支架内再狭窄的作用机制。方法:选用雄性新西兰兔50只,随机分为普通支架组及核素支架组,设正常对照。分别于术后3、7、14、28及56d取材,进行病理形态学、免疫组化(增殖细胞核抗原PCNA)、细胞凋亡(TUNEL)及原位杂交(bcl-2 mRNA及bax mRNA)的观察。结果:①光镜下发现,核素支架组管腔狭窄程度明显低于普通支架组,术后第56d最显著(P<0.01);②免疫组化显示,术后3-28d核素支架组PCNA表达均低于普通支架组,7d为表达高峰,16.35%±0.79%vs24.36%±0.55%(P<0.01)。③TUNEL法检测发现术后3-28d,核素支架组的平滑肌细胞凋亡较普通支架组更明显,术后第7d达峰值,14.72%±0.53%vs12.42%±1.13%(P<0.01)。④PCNA阳性率与TUNEL法测得的细胞凋亡阳性率比率PCNA/apoptosis(P∶A)显示,核素支架组P∶A的值在术后3-28d均显著小于普通支架组(P<0.05)。⑤原位杂交测定凋亡相关基因bcl-2 mRNA及bax mRNA表达并计算其比值显示,普通支架组术后第3、14、28d均大于对照组(P<0.01),核素支架组与对照组无显著差异(P>0.05)。术后第3-28d,核素支架组的比值均小于普通支架组(P<0.05)。结论:γ粒子核素支架通过抑制平滑肌细胞的增殖,促进凋亡,使增殖与凋亡的比率降低,从而减轻再狭窄的程度。AIM： To observe effect of γradioactive [ ^103 Pd ] stent on the proliferation and apoptosis of smooth muscle cells, the mechanism of radioactive stent preventing in - stent restenosis was explored. METHODS： Fifty male New Zealand rabbits were randomized into stent group and [ ^103 Pd ] stent group. Control group was set up. The materials were harvested on 3, 7, 14, 28, 56 days after operation and the following investigation were carried out, including pathomorphology, immunohistochemistry, apoptosis （TUNEL） and in situ hybridization studies. RESULTS： ① The severity of the stenosis in [ ^103 Pd ] stent group was less severe than that in stent group. It was most obvious on 56 th day （P 〈 0. 01 ）.②The expression of proliferating cell nuclear antigen（PCNA） of [ ^103Pd] stent group was lower than that in stent group on 3 to 28 days. It was most obvious on 7th day, 16. 35% ± 0. 79% vs 24. 36 ± 0. 55% （ P 〈 0. 01 ）.③ TUNEL method showed that the [ ^103Pd] stent group had much more apoptosis of VSMC than that in stent group. The highest rate of apoptosis appeared on day 7, 14. 72% ±0. 53% vs 12.42% ± 1.13% （P 〈0. 01 ）.④ By calculating the ratio of PCNA/apoptosis （P ： A）, a much lower ratio was seen in [^103Pd] - stent groups than that in stent group at 3 to 28 days. There was significant statistic difference （P 〈 0. 05 ）. ⑤ For bcl -2/bax ratio, the result in [^103pd ] - stent group was lower than that in stent group at 3 to 28 days. There was significant statistic difference （P 〈 0.05）. CONCLUSION：γradioactive stent inhibits the proliferation and accelerates apoptosis of injured media vascular smooth muscle cells. It decreases the ratio of proliferation to apoptosis and relieves the severity of restenosis.

关 键 词：放射性同位素 支架 细胞凋亡 血管平滑肌细胞 

分 类 号：R541.7[医药卫生—心血管疾病]