家兔“风门”穴皮内神经末梢感受器通路的研究  

Receptor closed circuit of intradermic nerve terminal at Fengmen acupoint in rabbits

作　　者：章云海[1] 耿祝生[1] 徐志强[1] 雷玲[1] 卢文[2] 李玉[3] 李静[1] 扬聪颖[4] 曾因明[5] 刘克芳[1] 

机构地区：[1]连云港市第一人民医院麻醉科,江苏省连云港市222002 [2]连云港市第一人民医院针灸科,江苏省连云港市222002 [3]连云港市第一人民医院肿瘤生物实验室,江苏省连云港市222002 [4]连云港市第一人民医院病理科,江苏省连云港市222002 [5]江苏省麻醉学重点实验室,江苏省徐州市221002

出　　处：《中国组织工程研究与临床康复》2007年第14期2680-2683,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30271260);江苏高校省级重点实验室第三批对外开放课题(K2093);江苏省科技厅指导计划课题(BS2002305);连云港市科技局课题(SH0408);连云港市科技局课题(SH0507)~~

摘　　要：目的:探讨家兔“风门”穴皮内神经末梢感受器通路与皮肤末梢感受器通路的关系,为中医皮部经络学说提供形态学依据。方法:实验于2005-10/11在连云港市第一人民医院肿瘤生物实验室进行。①选取雄性家兔4只,随机数字表法分为神经逆行追踪18～24h组、神经逆行追踪30～36h组,每组2只。②两组兔以乌拉坦1.5g/kg麻醉后,经双侧“风门”穴皮内注射荧光素核黄(Sigma,批号74681-68-8)1g/L(含核黄500μL)逆行神经追踪,神经逆行追踪18～24h组和神经逆行追踪30～36h组兔分别成活18～24h,30～36h后处死。③分别取C2～L5脊神经节、颈、胸交感神经节、肠系膜下神经节、大脑、小脑、丘脑、脑干、脊髓、胃、小肠、心肌、肺、肾、骶脊肌、背阔肌、膈肌、肠系膜下动脉、牵涉区部位的皮、骶部、“足三里”穴部的皮及耳,冰冻连续切片,分别做成两套,一套蒸馏水贴片,直接在Olympus ZX-004荧光显微镜下寻找各组织荧光标记的神经细胞、荧光标记密集的神经末梢部位;另一套行苏木精-伊红染色后再置于荧光显微镜下,观察有无新的发现。结果:4只兔均进入结果分析。①神经逆行追踪18～24h组:在C3～L3脊神经节发现标记细胞,以T3～12标记细胞较多;在颈、胸交感神经节及肠系膜下神经节发现标记细胞;在小肠黏膜及黏膜下标记到大量的节细胞;在脊髓后角、下丘脑的橄榄核、骶脊肌、背阔肌、膈肌的肌膜、心肌的内、外膜,胃、膀胱壁、肺支气管壁、肝管壁、胆囊壁的外膜和黏膜、肩关节滑膜、腹主动脉、肠系膜动脉壁的内、外膜、肾小管以及胃牵涉区、足三里穴的皮和骶部及耳的皮内毛细血管袢发现有荧光密集区。②神经逆行追踪30～36h组:C3～T2脊神经节核黄标记细胞大部分消失,T3～10仍较多,在颈、胸交感神经节、肠系膜下神经节及小肠节细胞的标记细胞与神经逆行追踪18～24h�AIM：To explore the relationship of the receptor closed circuit of intradermic nerve terminal at Fengmen acupoint in rabbits with the receptor closed circuit of cutaneous terminal, and provide the morphological base for skin channels and collaterals in traditional Chinese medicine. METHODS： The experiment was conducted in the Biochemical Laboratory of Tumor, Lianyungang First People＇s Hospital from October to November 2005. ①Four male rabbits were selected and randomly divided into retrograde neural tracing for 18-24 hours group and 30-36 hours group with 2 rabbits in each group. ②Rabbits of two groups were intradermically injected with 1 g/L fluorescent riboflavin containing 500 μL nuclear yellow （Sigma, No. 74681-68-8） at bilateral Fengmen acpoint under urethane （1.5 g/kg） anesthesia for retrograde neural tracing. The rabbits survived 18-24 hours or 30-36 hours in the 18-24 hours group or 30-36 hours group were sacrificed, respectively. ③The frozen serial sections were made in spinal ganglia from C2 to Ls, cervical and thoracic sympathetic ganglia, inferior mesenteric ganglion, cerebrum, cerebellum, thalamus, brain stem, spinal cord, stomach, small intestine, myocardium, lung, kidney, sacral spinal muscle, latissimus dorsi muscle, diaphragmatic muscle, inferior mesenteric artery, dermis in the referral area, pars sacralis, dermis at Zusanli acpoint and ear, respectively. The sections were made into 2 series： one was stuck in the distilled water, then observed under an Olympus ZX-004 fluorescence microscope directly to find fluorescence marked neuron and fluorescence dense area of nerve terminal; the other was stained with hematoxylin and eosin, then observed under the fluorescence microscope to determine whether there was new discovery. RESULTS： Four rabbits were involved in the result analysis. ①In the retrograde neural tracing 18-24 hours group, the labeled cells were found in spinal ganglia C3 -L3, and more cells in T5-T12; the labeled cells were also found in cervical and thora

关 键 词：“风门”穴 皮内 感受器通路 脊神经节 交感神经节 小肠节细胞 下丘脑 

分 类 号：R441.1[医药卫生—诊断学]