PTPases抑制剂对外源NO调控蚕豆气孔运动的影响  

Effects of PTPases Inhibitor on Exogenous NO Regulated Stomatal Movement of Vicia faba L.

作　　者：李晓磊[1] 石武良[2] 车永梅[1] 刘新[1] 

机构地区：[1]莱阳农学院生命科学学院,山东青岛266109 [2]中国科学院植物研究所中国科学院光合作用与环境分子生理学重点实验室,北京100093

出　　处：《西北植物学报》2007年第3期620-624,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：山东省教育厅基金(J04C13);国家自然科学基金(30370141)资助

摘　　要：以蚕豆（Vicia faba L.）气孔保卫细胞为材料,研究了酪氨酸蛋白磷酸酶（protein tyrosine phosphatases,PTPases）的抑制剂氧化苯胂（phenylarsine oxide,PAO）、钒酸钠（NaVO3）和Zn2＋对外源一氧化氮（NO）调控蚕豆气孔运动的影响.结果表明,NO供体硝普钠（sodium nitroprusside,SNP）能诱导蚕豆气孔关闭,其效应在0.001～0.1 mmol·L^-1浓度范围内随着SNP浓度的增大而增强;不同浓度的PAO、NaVO3和Zn2＋对光诱导的气孔张开几乎没有影响,但都可以抑制黑暗或SNP诱导的气孔关闭,表明酪氨酸蛋白磷酸酶参与NO调控蚕豆气孔运动的信号转导过程,在NO调控蚕豆气孔运动中起着重要的作用.The effects of protein tyrosine phosphatases （PTPases） inhibitor phenylarsine oxide （phenylarsine oxide,PAO）,sodium vanadate （NaVO3） and Zn^2＋ on exogenous NO regulated Vicia faba L. stomatal movement were studied. The results showed that SNP （sodium nitroprusside）, the donor of NO, can induce Vicia faba L. stomatal closure when SNP density was between O. 001 mmol · L^-1 and 0. 1 mmol · L^-1. The effect was enhanced along with SNP density increasing. PAO,NaVOa and Zn^2＋ of different density had no effects on light induced stomatal opening,but inhibited darkness or SNP induced stomatal closure. The results suggested that PTPases participated in the signal transduction of NO regulated Vicia faba L. stomatal movement,PTPases played a vital role in the NO regulated Vicia faba L. stomatal movement.

关 键 词：酪氨酸蛋白磷酸酶 NO 蚕豆 气孔运动 

分 类 号：Q945.78[生物学—植物学]