罗格列酮对高脂血症大鼠拮抗胰岛素抵抗及脂肪性肝损伤作用(英文)  

Effects of rosiglitazone on antagonizing insulin resistance and adipose liver damnification in hyperlipemic rats

作　　者：郑琳颖[1] 潘竞锵 林洁茹[1] 肖柳英 李博萍[1] 韩超 

机构地区：[1]广州市中医医院药剂科,广东省广州市510130 [2]广州市中医中药研究所药理研究室,广东省广州市510130

出　　处：《中国组织工程研究与临床康复》2007年第16期3189-3192,3200,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:有实验表明在胰岛素缺乏的糖尿病动物应用罗格列酮并未发现明显的增加胰岛素和降血糖作用,说明该药不刺激胰岛素分泌,其改善胰岛素抵抗的作用及对肝、肾脏功能的影响有待研究。目的:观察罗格列酮对高脂血症大鼠胰岛素抵抗是否有改善作用并分析其可能的作用机制。单位:广州市中医医院,广州市中医中药研究所。设计:分层随机对照动物实验。材料:选用64只SD大鼠,鼠龄6-8周,雌雄各半,体质量150-180g,均购自广东省医用实验动物中心;基础饲料:总热量6.9kJ/g(其中蛋白质占23%,碳水化合物占53%,脂肪占5%)。高脂乳液:猪油200g/L,胆固醇200g/L,牛胆盐10g/L,丙二醇200g/L,吐温-80200g/L,总热量15.5kJ/g。高脂肪-高糖-高热量饲料:基础饲料加100g/L葡萄糖、200g/L猪油和100g/L蛋黄粉充分混匀后,制成饼块,烘干后用,总热量21.3kJ/g(其中蛋白质占15%,碳水化合物占51%,脂肪占30%);罗格列酮片:葛兰素史克(天津)有限公司生产(5mg/tab,批号:02110012);格列齐特片:法国施维雅药厂天津华津制药厂合作生产(100mg/tab,批号:00232)。方法:实验于2003-04/07在广州中医药大学完成。①按性别、体质量随机抽取16只大鼠作为空白对照组,喂饲普通饲料,共6周。其余大鼠按照参照文献方法灌服高脂乳液,取空腹血糖≥6.1mmol/L或2h血糖≥7.8mmol/L的大鼠,按体质量和血糖值将大鼠分成3组:模型组、罗格列酮组和格列齐特组,每组16只。空白对照组大鼠不给予处理。罗格列酮组及格列齐特组大鼠分别灌胃给予罗格列酮(5mg/kg)及格列齐特(100mg/kg),模型组灌胃蒸馏水,同时分别继续喂饲高脂饲料,1次/d,共28d,第21天开始同时灌服高脂乳液,1次/d,共7d,末次给药后,禁食18h,第29天采用血糖仪检测各组大鼠空腹血糖,按体质量分别灌胃2.78mol/10mL·kg葡萄糖溶液或葡萄糖-药物混合液10mL/kg,2h后测2h血糖。②全部大鼠眼眶放血并分离�BACKGROUND： Some experiments indicated that applying rosiglitazone on diabetic animals lacking of insulin could not increase insulin and lower blood glucose obviously, which showed that rosiglitazone did not stimulate the excretion of rosiglitazone. The action of rosiglitazone in improving insulin resistance and the effects on the functions of liver and kidneys need more investigations. OBJECTIVE： To investigate whether rosiglitazone can improve the insulin resistance of rats with hypedipemia, and analyze the possible mechanism. SETTINGS： Guangzhou Hospital of Traditional Chinese Medicine; Guangzhou Institute of Traditional Chinese Medicine and Materia. Medica DESIGN ： A stratified randomized controlled animal trial. MATERIALS ： Sixty-four Sprague-Dawley （SD） rats （Batch No. 2002A024）, SPF grade, half male and half female, weighing 150 to 180 g, aged 6 to 8 weeks were purchased from Guangdong Medical Experimental Animal Center. Normal feed （total quantity of heat 6.9 kJ/g） was enriched with 23% protein, 53% carbohydrate and 5% fat. High fat emulsion （total quantity of heat 15.5 kJ/g） was enriched with 200 g/L lard, 200 g/L cholesterol, 10 g/L bile salt ox, 200 g/L propylene glycol, 200 g/L tween-80. High fat and sugar feed （total quantity of heat 21.0 kJ/g） was enriched with 15% protein, 51% carbohydrate and 30% fat after adding 100 g/L glucose, 200 g/L lard and 100 g/L yolk powder then mixing and baking. Rosiglitazone was from GlaxoSmithKline Co Ltd. （Tianjin） （5 mg/tab, Batch No.02110012）. Gliclazide was from Servier International and Tianjin Hua Jin Pharmaceutical Factory （100 mg/tab, Batch No.00232）. METHODS ： The experiment was carried out in Guangzhou University of Traditional Chinese Medicine from April to July in 2003. （1） Sixty-four Sprague-Dawley rats, 16 of which were randomly sampled as the normal control group and had been fed with normal feed for 6 weeks. The others were modeled after medical literatures, each one was administered with high fat

关 键 词：高脂血症 胰岛素 大鼠 格列齐特 罗格列酮 胰岛素抵抗 

分 类 号：R458.5[医药卫生—治疗学]