快速老化鼠和自然衰老鼠中超氧化物歧化酶的比较研究  

The comparison of SOD expression between senescence-accelerated and natural aging mice

作　　者：李国星[1] 刘克明[1] 何宁[1] 王春花[1] 张明月[1] 

机构地区：[1]天津市卫生防病中心,天津300011

出　　处：《中国老年学杂志》2007年第9期820-822,共3页Chinese Journal of Gerontology

基　　金：天津市自然科学基金资助项目(No.023612011)

摘　　要：目的通过比较快速老化鼠(SAM-P/8)和昆明种自然衰老鼠中超氧化物歧化酶(SOD)表达情况,探讨两种衰老鼠之间异同及SOD在衰老过程中所起的作用。方法选用快速老化鼠(SAM-P/8)3月龄和6月龄各10只,昆明种小鼠3月龄和15月龄各10只,采用生物化学方法检测SOD蛋白活性和MDA含量;免疫组化对SOD蛋白的表达定位;蛋白印迹法检测SOD蛋白的含量变化;RT-PCR方法检测SOD基因的表达变化。结果两种衰老小鼠肝脏中SOD基因表达,SOD蛋白表达和活性均呈增龄性降低,MDA含量均呈现增龄性增加。结论快速衰老鼠和自然衰老鼠的SOD变化趋势相同,SOD在衰老过程中起了重要作用。Objective To explore the role of superoxide dismutase （SOD） in aging course and the difference between celerated and＇Kunming aging mice by the detection of SOD expression. Methods 20 senescence-accelerated mice （ 10 three-month and 10 six-month old mice）and 20 Kunming mice （10 three-month and 10 fifteen-month old mice）were selected to detect the location of SOD by immunohistochemistry, SOD protein activity and MDA content by biochemistry, SOD protein content by western blot and SOD gene changes by RT-PCR. Results The expression and activity of SOD protein and gene in liver obviously decreased in aging models. MDA content presented aging increase. Condusions SOD shows similar changes trend between senescence-accelerated and Kunming natural aging mice, playing an important role in aging.

关 键 词：快速老化鼠 衰老 超氧化物歧化酶 

分 类 号：Q3[生物学—遗传学]