丹参单体对肿瘤坏死因子损伤的血管平滑肌细胞炎症介质IL-1β表达的影响  

The influence of danshen root monome on the expression of inflammation mediator IL-1β of vascular smooth muscle cell induced by TNF-α

作　　者：聂桂丽[1] 郭茂娟[1] 李燕平[1] 范英昌[1] 

机构地区：[1]天津医学高等专科学校,天津300222

出　　处：《中国老年学杂志》2007年第9期823-824,共2页Chinese Journal of Gerontology

基　　金：天津市自然科学基金项目(023611611)

摘　　要：目的探讨丹参主要单体成分丹酚酸B和丹参酮IIA在动脉粥样硬化(AS)发病过程中发挥作用的物质基础和作用靶点。方法通过体外培养大鼠主动脉平滑肌细胞,建立肿瘤坏死因子(TNF-α)损伤的细胞模型,并运用放射免疫方法,检测上清液中IL-1β的表达。结果高剂量丹参酮IIA对IL-1β表达抑制作用最强(P<0.01),其余中药组抑制作用次之(P<0.05)。结论丹酚酸B和丹参酮IIA均可以通过抑制AS时炎症介质的表达达到防治AS的目的。Objective To explore the operative materials and the different target of salvianolic acid B （Sal B） and tanshinone ⅡA （TanⅡA） extracted from danshen root in atherosclerosis. Methods The aortic smooth muscle cells were cultured in vitro to establish TNF-α induced cell model and to detect IL-1β expressions by radioimmunity. Results The high TanlIA group markedly decreased the expression of IL-1β P 〈 0.01 ） , other groups also had some effect（ P 〈 0.05 ）. Conclusions Both salvianolic acid B and tanshinone IIA could prevent and treat atherogenesis by inhibiting the expression of IL-1β.

关 键 词：丹参单体 动脉粥样硬化 平滑肌细胞:肿瘤坏死因子 白介素-1 

分 类 号：R541.4[医药卫生—心血管疾病] R96[医药卫生—内科学]