Mitochondrial DNA analysis of Bronze Age horses recovered from Chifeng region, Inner Mongolia, China  

作　　者：Cai Dawei Han Lu Xie Chengzhi Li Shengnan Zhou Hui Zhu Hong 

机构地区：[1]Ancient DNA Laboratory, Research Center for Chinese Frontier Archaeology of Jilin University, Changchun 130023, China [2]College of Life Science, Jilin University, Changchun 130023, China

出　　处：《Progress in Natural Science:Materials International》2007年第5期544-550,共7页自然科学进展·国际材料（英文版）

基　　金：Supported by National Science Fund for Fostering Talents in Basic Research (Grant No. J0530184);National Social Science Foundation of China (Grant No. 06BKG001)

摘　　要：In this study, mitochondrial DNA （mtDNA） analysis was carried out on 9 Bronze Age horses recovered from Dashanqian and Jinggouzi archaeological sites in Chifeng region, Inner Mongolia, China to explore the origin of Chinese domestic horses. Both mtDNA 16S rRNA gene and control region （D-loop） fragments of ancient horses were amplified and sequenced. The analysis of the highly conservative 16S rRNA gene sequences indicated that the burial environment of Chifeng region is suitable for the preservation of ancient DNA （aDNA）. Combing 465 mtDNA D-loop sequences representing different breeds from East Asia, Central Asia, Near East and Europe, we constructed a phylogenetic network to investigate the relationship between ancient and modern horses. The phylogenetic network showed that the 9 horses were distributed into different modern horse clusters which were closely related to them representing a certain geographical distribution. Our results showed that the maternal genetic line of the ancient horses in Chifeng region was highly diversified, which contributed to the gene pool of modern domestic horses and suggested a complex origin of domestic horses in China.在这学习 mitochondrial DNA (mtDNA ) ，分析在马在 Chifeng 区域从 Dashan-qian 和 Jinggouzi 考古学的地点恢复了的 9 青铜时代上被执行，内部蒙古，探索中国国内马的起源的中国。古老的马的两 mtDNA 16S rRNA 基因和控制区域(D 环) 碎片被放大并且定序。高度保守的 16S rRNA 基因序列的分析显示 Chifeng 区域的埋葬环境对古老的 DNA (aDNA ) 的保藏合适。梳 465 mtDNA D 环定序从东亚，中亚，近的东方和欧洲代表不同品种，我们构造了一个种系发生的网络调查在古老、现代的马之间的关系。种系发生的网络证明 9 匹马与他们有关仔细是分布式的进是的不同的现代的马簇代表某个地理分发。我们的结果证明在 Chifeng 区域的古老的马的母亲的基因线是高度多样化的，它贡献了现代的国内马的基因水池并且在中国建议了国内马的复杂起源。

关 键 词：origin of domestic horses ancient DNA mitochondrial DNA 16S rRNA gene phylogenetic network 

分 类 号：Q915.877[天文地球—古生物学与地层学] Q959.843[生物学—古生物学]