Mutagenic breeding of silver-resistant Acidithiobacillus ferrooxidans and exploration of resistant mechanism  

作　　者：吴学玲 邱冠周 高健 丁建南 康健 刘新星 

机构地区：[1]Key Laboratory of Biometallurgy of Ministry of Education, School of Resources Processing and Bioengineering, Central South University

出　　处：《中国有色金属学会会刊：英文版》2007年第2期412-417,共6页Transactions of Nonferrous Metals Society of China

基　　金：Project(5032142) supported by Chinese Science Foundation for Distinguished Group;Project(2004CB619201) supported by the Major State Basic Research Development Program of China;Project(50374076) supported by National Natural Science Foundation of China

摘　　要：The silver-resistant Acidithiobacillus ferrooxidans were isolated from 22 acid mine drainage (AMD) samples collected from Dexing Copper Mine and Chengmen Mountain Mine, Jiangxi Province, China. Isolate DX16 is obtained from the sample taken from Dexing Copper Mine and still carries out ferrous ion oxidation when incubated in 9K medium containing silver nitrate (240 mg/L). While isolate H1, a less resistant strain taken from Yin Mountain Mine, has a tolerate level of only 60 mg/L. Based on 16S ribosomal DNA sequencing, both bacterial 16SrDNA sequences are 100% similar to Acidithiobacillus ferrooxidans ATCC 23270. Through ultraviolet irradiation induced mutations, isolate mDX16 that is obtained from DX16 carries out ferrous ion oxidation when incubated in 9K medium containing higher concentration of silver nitrate (250 mg/L). When silver-resistant gene (SilC) analysis is carried out on the two isolates, it is seen that this gene was absent in both.The silver-resistant Acidithiobacillus ferrooxidans were isolated from 22 acid mine drainage （AMD） samples collected from Dexing Copper Mine and Chengmen Mountain Mine, Jiangxi Province, China. Isolate DX16 is obtained from the sample taken from Dexing Copper Mine and still carries out ferrous ion oxidation when incubated in 9K medium containing silver nitrate （240 mg/L）. While isolate HI, a less resistant strain taken from Yin Mountain Mine, has a tolerate level of only 60 mg/L. Based on 16S ribosomal DNA sequencing, both bacterial 16SrDNA sequences are 100% similar to Acidithiobacillus ferrooxidans ATCC 23270. Through ultraviolet irradiation induced mutations, isolate mDX16 that is obtained from DX16 carries out ferrous ion oxidation when incubated in 9K medium containing higher concentration of silver nitrate （250 mg/L）. When silver-resistant gene （SilC） analysis is carried out on the two isolates, it is seen that this gene was absent in both.

关 键 词：嗜酸氧化亚铁硫杆菌 银耐受性 突变育种 耐受机理 生物浸矿 

分 类 号：TG174[金属学及工艺—金属表面处理]