轮状病毒不同毒株感染宿主细胞的感染效力研究  

Comparative studies of the infection of different rotavirus to host cell

作　　者：何海洋[1] 李晋涛[1] 费蕾[1] 姜曼[1] 牟芝蓉[1] 付晓兰[1] 唐艳[1] 段召军[2] 吴玉章[1] 

机构地区：[1]第三军医大学基础部全军免疫学研究所,重庆400038 [2]中国疾病预防控制中心病毒病预防控制所,北京100050

出　　处：《免疫学杂志》2007年第3期255-259,共5页Immunological Journal

基　　金：国家自然科学基金(30400402;30400437和30571708)资助

摘　　要：目的比较轮状病毒Wa株和SA11株对MA104细胞的感染及复制能力,研究不同毒株对宿主细胞的感染效力。方法差速离心浓缩病毒颗粒,FFA法检测病毒滴度,流式细胞术检测病毒对MA104细胞的感染效率,ELISA检测不同病毒颗粒浓度,并比较2毒株间异同。结果Wa株感染效率随病毒量的变化明显快于SA11株,极限感染效率也较SA11高;2毒株病毒产量均随感染病毒用量增加而明显升高,而感染了SA11细胞的平均病毒生成能力显著高于感染Wa株的细胞。结论轮状病毒Wa株与SA11株感染细胞效力差异显著(P<0.05),为进一步研究轮状病毒感染宿主细胞及与之相互作用特点提供了实验依据。Objective To compare the infectivity and reproductive capability in MA104 cells of different rotavirus strains Wa and SA11, and analyze their differences in infection, Methods The rotavirus was prepared by differential centrifugation, and the virus liters were detected by FFA. Flow cytometry was used to detect virus infectivity to MA104 cells, ELISA was employed to detect rotavirus particle amounts and analyze the differences between the two rotavirus strains. Results The infective ability of Wa to MA104 cells was obviously higher than thatofrotavirusSA11, while the cells infected by SA11 have higher virus productivity than those infected by Wa strain. Conclusion Rotaviruses Wa and SA11 has obviously different infection pattern. The results give a basis for studying the properties of rotavirus interaction with host cells.

关 键 词：轮状病毒 病毒感染率 病毒复制能力 流式细胞术 荧光集落分析法 

分 类 号：R373.9[医药卫生—病原生物学]