Ce(NH_4)_2(NO_3)_6对蔓茎堇菜悬浮细胞凋亡和总黄酮含量的影响  

Effect of Ce(NH_4)_2(NO_3)_6 on apoptosis and content of total flavonoids of suspension cultured cells of Viola diffusa Ging.

作　　者：代容春[1] 陈由强[1] 黄儒珠[1] 洪丽亚[1] 

机构地区：[1]福建师范大学生命科学学院,福建福州350007

出　　处：《植物资源与环境学报》2007年第1期23-27,共5页Journal of Plant Resources and Environment

基　　金：福建省自然科学基金资助项目(B0510009)

摘　　要：用DNA Laddering和DAPI荧光检测法对Ce(NH4)2(NO3)6诱导的蔓茎堇菜(Viola diffusa Ging.)悬浮细胞的凋亡情况进行研究,同时还测定了诱导过程中悬浮细胞的生长量和总黄酮含量。结果表明,Ce(NH4)2(NO3)6可诱导蔓茎堇菜悬浮细胞发生凋亡,1.0 mmol.L-1Ce(NH4)2(NO3)6胁迫诱导培养8 d时,细胞凋亡率最高(55.7%);总黄酮含量显著增加,达2.820%。表明Ce(NH4)2(NO3)6在诱导蔓茎堇菜悬浮细胞凋亡并抑制其生长的同时,还能促进黄酮类化合物的合成。The apoptosis of suspension cultured cells of Viola diffusa Ging. which had been induced by Ce（NH4）2（NO3）6 were detected by DNA Laddering and DAPI fluorescence stain methods. Cell growth and content of total flavonoids in suspension cultured cells were also determined. The results showed that different concentrations of Ce（NH4）2（NO3）6 could induce apoptosis of suspension cultured cells, and the rate of apoptosis was the highest （55.7%）and content of total flavonoids reached to 2. 820% when they were induced by 1.0 mmol·L^-1 Ce（NH4）2（NO3）6 for eight days. It was indicated that Ce（NH4）2（NO3）6 can induce apoptosis and inhibit cell growth, it also can promote the synthesis of flavonoids in suspension cultured cells of V. diffusa.

关 键 词：蔓茎堇菜 硝酸铈铵 细胞凋亡 总黄酮 悬浮培养 

分 类 号：Q945.14[生物学—植物学]