红豆杉细胞培养中紫杉醇高产细胞株的筛选及其稳定性分析  

Selection and stability research on taxol high-yield cell strain during Taxus cell culture

作　　者：李志良 李干雄 饶秋容 黄巧明 曾腾锋 

机构地区：[1]梅雁生物工程研究所,广东梅州514787

出　　处：《植物资源与环境学报》2007年第1期62-65,共4页Journal of Plant Resources and Environment

基　　金：广东省科学技术厅工业攻关项目(2005B10401032)

摘　　要：对中国红豆杉〔Taxus chinensis(Pilger)Rehd.〕、云南红豆杉(T. yunnanensis Cheng et L.K.Fu)和东北红豆杉(T. cuspidata Sieb.et Zucc.)不同部位的愈伤组织进行培养及分析比较。结果表明,中国红豆杉愈伤组织生长较快,其叶片诱导的愈伤组织紫杉醇含量较高,且紫杉醇含量与培养物的外观特征有明显相关性,颜色浅、块状或颗粒较明显的细胞团紫杉醇含量较高。运用细胞看护培养技术,从中国红豆杉愈伤组织中筛选出生长速率达0.52g.L-1.d-1、紫杉醇含量超过0.01%的细胞优株,经20次继代培养,其生长和紫杉醇含量均较稳定。Calli from different parts of Taxus chinensis （Pilger） Rehd. , T. yunnanensis Cheng et L. K. Fu and T. cuspidata Sieb. et Zucc. were induced and analyzed. The results showed that callus growth of T. chinensis was quicker, taxol content of callus which induced from leaves of T. chinensis was higher, and taxol content had a relationship with external characteristics of the callus. The cell aggregate which lumped up or obvious granulate and the color was shallow had higher content of taxol. Using technology of cell nurse culture, the excellent cell strain which growth rate reached to 0.52 g·L^-1·d^-1 and taxol content was more than 0.01%, was selected from callus of T. chinensis, and its growth and taxol content were more stable after subcuhured twenty times.

关 键 词：红豆杉 紫杉醇 高产细胞株 看护培养 

分 类 号：S567.19[农业科学—中草药栽培] Q943.1[农业科学—作物学]