萱草花粉类动力蛋白生物物理学及药理学性质(英文)  

Biophysical and Pharmacological Characterization of a Dynamin-like Protein from Day-lily (Hemerocallis fulva, Liliaceae) Pollens

作　　者：廖俊杰[1] 吴英杰 阎隆飞[1] 

机构地区：[1]中国农业大学生物学院植物生理生化国家开放重点实验室 [2]Department of Medicine,Mount Sinai School of Medicine,New York,NY10029

出　　处：《云南植物研究》2007年第2期247-250,258,共5页Acta Botanica Yunnanica

基　　金：This work was supported by the National Science Fund (No.39730280)

摘　　要：利用FPLC技术从萱草花粉中鉴定并纯化了动力蛋白,研究了它的酶学性质及部分生物化学性质。结果如下:纯化的类动力蛋白分子量为100kD,等电点pI=6·15和6·80。在280nm波长激发下,最大的荧光发射波长是346nm。荧光光谱分析结合紫外吸收光谱及导数光谱分析推断它含有色氨酸和酪氨酸残基。药理学性质研究表明巯基可能在酶的活性中心起重要作用。A novel motor protein, dynamin-like protein from day lily, was purified and identified by FPLC. Here we report its biochemical characterization. The molecular weight of the dynamin-like protein is 100 kD on SDS-PAGE. Isoelectric points are about 6.15 and 6.80. The fluorescence emission wave length of dynamin-like protein is 346 nm by excitation at 280 nm. Through fluorescence spectra analysis, ultraviolet absorption spectrum and derivative spectrum, we conclude that it contains tryptophan and tyrosine residues. Pharmacological study indicates that mercapto may play an important role in etienne activity of dynamin-like protein.

关 键 词：类动力蛋白 分子马达 微管 花粉 

分 类 号：R285[医药卫生—中药学]