从大鼠肺灌洗液中分离纯化培养卡氏肺孢子虫  

Continuous Axenic Cultivation of Pneumocystis carinii Isolated from the Bronchoalveolar Lavage Fluid of Infected Rat

作　　者：黄敏君[1] 安亦军[1] 李淑珍[1] 卢思奇[2] 郭增柱[1] 

机构地区：[1]首都医科大学附属北京友谊医院北京热带医学研究所,北京100050 [2]首都医科大学寄生虫学教研室,北京100089

出　　处：《中国寄生虫学与寄生虫病杂志》2007年第2期129-132,136,共5页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：北京市自然科学基金项目(No.7052009)~~

摘　　要：目的建立卡氏肺孢子虫(Pneumocystis carinii,P．c)纯培养株。方法从肺孢子虫肺炎(PCP)大鼠模型离体肺脏的灌洗液中分离P.c,用改良IMDM培养基进行体外培养;以四胺银染色计数法观察虫体增殖情况;用PCR扩增培养物中P．c线粒体大亚基rRNA基因,进行基因鉴定;用透射电镜观察培养虫体的超微结构。结果用添加s-腺苷甲硫氨酸(SAM)等辅助剂的IMDM培养基从8只PCP大鼠的肺灌洗液中分离出5个Pc纯培养株(P.c Rat1-5)。分离培养的P.c可进行冷冻保存和复苏培养。培养72 h的P.c包囊可增殖19～22倍。形态、超微结构及基因序列分析证实从大鼠肺灌洗液中分离出的培养物是大鼠源性肺孢子虫。结论从大鼠肺灌洗液中分离培养出5株大鼠源卡氏肺孢子虫。Objective To establish axenic cultivation of Pneumocystis carinii （P.c）. Methods The organisms of P.c were isolated from the bronchoalveolar lavage fluid （BALF） of the rats with Pneumocystis carinii pneumonia （PCP） and cultured in a medium which was based on IMDM（GIBCO） supplemented with S-adenosyl-L-methionine, putrescine, N-acetyl glucosamine, putrescine, L-cysteine and L-glutamine, and newborn calf serum. The organisms cultured in the system were identified by observing the morphology of cysts in smears stained with Gomori＇s methenamine silver nitrate stain （GMS）. Uhrastructure of the cysts/trophozoites was examined by transmission electron microscopy. The sequences of mitochondrial large ribosomal DNA subunit of the cutured organisms were compared with the Pneumocysti carinii f.sp. ratti variant isolate （GenBank No U20173） and Pneumocystis carinii f.sp.hominis （GenBank No M58605）. Results Five isolates of P.carinii received from BALF of 8 rats with PCP were cultured axenically and continuously in the system. The cultured organisms could be stored in frozen condition and used to reinitiate culture, and were amplified by 19-22 times within 72 h. The morphology, uhrastructure and gene sequencing of the cultured organisms confirmed that the isolated organisms were P.carinii. Conclusion Five continuously and axenicly cultured isolates of P.carinii have been received.

关 键 词：卡氏肺孢子虫 培养 分离株 

分 类 号：R382.33[医药卫生—医学寄生虫学]