猪外周血单个核细胞和植物血凝素对猪淋转试验的影响  

Effect of Peripheral Blood Monocyte of Big and Plant Prothrombin on Lymph Transform Test of Pig

作　　者：张佩君[1] 

机构地区：[1]陕西教育学院,陕西西安710061

出　　处：《陕西教育学院学报》2007年第1期88-90,111,共4页Journal of Shaanxi Institute of Education

基　　金：陕西教育学院科研基金项目(06KJ023)

摘　　要：选用健康种猪18头,按1、2、3月龄分为3组,每组6头,无菌采取前腔静脉血10 mL,肝素抗凝,分离外周血单个核细胞（PBMC）,计数并稀释成1×10^7个/mL、5×10^6个/mL、2.5×10^6个/mL、1.25×10^6个/mL、6.25×10^5个/mL五个浓度梯度;植物血凝素-p（PHA-P）配成100μg/mL、50μg/mL、25μg/mL、12.5μg/mL、6.25μg/mL五个浓度梯度.采用正交试验法在96孔细胞培养板中加入不同浓度的PBMC和PHA-p,用MTT法检测淋转试验结果.三组试验结果均表明PBMC和PHA-p对猪淋巴细胞增殖反应均有显著的影响（0.01＜p＜0.05）,其最佳反应条件组合为：PBMC浓度为1.25×10^6个/mL,PHA-p浓度为12.5μg/mL,同时也表明年龄大小不影响猪的淋转试验.The paper choices 18 caput of health boardistinguishedinto 3 groups by different month （one month, two month, three month）.Every group includ 6 caput of health boar; asepsis adopting blood of vein 10ml in former celiac, adding anticoagulant, separating peripheral blood monocyte （PBMC）, taking count of PBMC and diluting different 5 grads of chroma （1×10^7 individual/ml, 5×10^6 individual/ml, 2.5×10^6 individual/ml, 1.25×10^6 individual/ml, 6.25×10^5 individual/ml）; plant prothrombin-p （PHA-p） was matched different 5 grads of chroma （100μ g/ml, 50μ g/ml, 25μ g/ml, 12.5μ g/ml, 6.25μ g/ml）. Adopting orthogonal test PBMC and PHA-p of different chroma entered into 96 apertures cell-cultivated board, deteeting result of lymph transform test by means of MTT. 3 groups of test result indicate： PBMC and PHA-p significantly influenced increment reactivity of lymphocyte of pig （0.01〈p〈0.05）, that optimal condition of reactivity： chroma of PBMC is 1.25×10^6 individual/ml, ehroma of PHA-p is 12.5μ g/ml, simultaneity, age magnitude didn＇t influence lymph transform test of pig.

关 键 词：猪PBMC PHA-p 淋转试验 

分 类 号：Q461[生物学—生理学]