血小板抗体检测方法的比较及其临床应用  

Comparison with the methods of platelet antibodies measurement and its clinical application

作　　者：张颖琪[1] 陈钰[2] 夏文权[2] 张利伟[2] 邵慧珍[2] 王鸿利[2] 

机构地区：[1]上海市第七人民医院,200137 [2]上海第二医科大学瑞金医院,200137

出　　处：《上海医学》1997年第2期95-97,共3页Shanghai Medical Journal

摘　　要：单克隆抗体固相化（MAIPA）、抗心磷脂抗体检测（ACA）、狼疮抗凝物质检测（LA）、酶联免疫吸附竞争法对ITP、输注血小板悬液和非ITP血小板减少症患者进行了抗血小板抗体的检测比较。结果表明，在ITP中MAIPA法的特异性显著高于酶联免疫吸附竞争法（P＜0．05）。在输注单产血小板悬液的6例患者中，均于输后第2、3周PAIgG明显升高。ACA检测在ITP组中的阳性率为23％，LA在ITP组和白血病组中的阳性率分别为9％、20％。最后又对两种方法的比较进行评价及血小板抗体检测的临床应用进行讨论。Platelet antibodies on patients with ITP,platelet suspension transfusion and non - ITP thrombocytopeniawere measured by using monoclonal antibody-specific immobilization of platelet antigen (MAIPA). anticardialipid anti-bodies (ACA) and lupus anticoagulant (LA)measurement and competing-ELISA. The specific of MAIPA was greatlyheder than competing-ELISA on patients with ITP (P<0. 05). in 6 patients with single producing platelet suspensiontrans fusion,we found their PAIgG were much higher within 2~3 weeks after transfusion. The positive rate of ACA onITP group was 23%,the positive rate of LA on ITP group and leukemia group were 9% and 20% respectively. Finally,we evaluated the comparison with two methods and discussed the clinical application of platelet antibody's measurement.

关 键 词：血小板 抗体检测 单克隆抗体 固相比 ELISA 

分 类 号：R446.1[医药卫生—诊断学]