患者的ELISA一步法检测乙型肝炎抗-HBc、抗-HBe存在假阳性  

Study of false positive reaction in determination of serum anti-HBc and anti-HBe by one-step ELISA

作　　者：黄作美[1] 黄光华[1] 陈江萍[1] 

机构地区：[1]江西省肿瘤医院,330029

出　　处：《上海医学》1997年第2期100-102,共3页Shanghai Medical Journal

摘　　要：为证实酶联免疫吸附试验（ELISA）一步法检测抗-HBc和抗-HBe之间的相互交叉反应，用国产试剂对166例临床标本及266例体检标本，采用一步法和两步法检测并与Abbott产品结果比较发现其相对特异性，抗-HBc依次为50％、67．7％和98．2％、97．5％。抗-HBe依次为89．3％、88．6％和98．8％、99．2％，一步法均明显低于两步法。将两法结果不符的再与Abbott产品结果比较，抗-HBc检测临床标本存在30％和体检标本存在16％的差异，抗-HBe也分别存在12．2％和11．9％的差异，这些差异主要是试剂特异性低引起的非特异性交叉反应。因此一步法检测抗-HBc和抗-HBe存在假阳性。To certify the higher false positive reaction rate of serum anti-HBc and anti-HBe, 166 clinical specimensand 266 physical examing specimens were detected by one-step and two-step Enzyme Linked Immuno Sorbent Assay(ELISA) respectively with national reagents. Besides,the ABBOTT reagent was also used. Calculated by comparing thetwo reagents,the relative specificity of 166 clinical specimens and 266 physicaI examing specimens were 50K and 67. 7%for anti-HBc, 89. 3% and 88. 6% f0r anti-HBe. That determined by two-step ELISA were 98. 2% and 97. 5% for anti-HBc, 98. 8% and 99- 2% for anti-HBe respectively. The deference between national reagents and the ABBOTT reagentwere 30% in clinical specimens and 16% in physical examing specimens for anti-HBc, 12. 2% and 11. 9% for anti-HBe.The relative specificity of one-step is lower than that of two-step method,There is higher fales positive reaction in de-tecting anti-HBc and anti-HBe by one-step ELISA.

关 键 词：乙型肝炎病毒 抗-HBC 抗-HBE 假阳性 ELISA 

分 类 号：R512.620.4[医药卫生—内科学]