胺碘酮和维拉帕米对体外激活的淋巴细胞分泌细胞因子的影响  

Effects of Amiodarone and Verapamil on the production of cytokine of in intro activated lymphocytes

作　　者：王宪沛[1] 廖玉华[1] 胡钢[2] 邹安若[1] 李璐[1] 袁璟[1] 涂丹娜[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院,湖北武汉430022 [2]荆州市中心医院

出　　处：《山东医药》2007年第13期10-11,共2页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30470711)。

摘　　要：目的研究胺碘酮(AMI)和维拉帕米(VER)对体外脂多糖(LPS)激活的人外周血淋巴细胞分泌细胞因子的影响,初步探讨此类药物发挥免疫调节作用的机理。方法AMI和VER作用于LPS激活的人外周血淋巴细胞,用双抗体夹心酶联免疫吸附法检测培养液上清中细胞因子白介素2(IL-2)、肿瘤坏死因子α(TNF-α)、白介素10(IL-10)和转化生长因子β1(TGF-β1)水平。结果AMI和VER均可浓度依赖性抑制IL-2、TNF-α和TGF-β1的分泌,提高IL-10的分泌。结论AMI和VER通过对体外淋巴细胞的直接作用调节细胞因子的产生,淋巴细胞膜离子通道可能为其作用靶点之一。[Objective] To investigate the effects of Amiodarone and Verapamil on the production of cytokine in lipopolysaccharide （LPS）-induced human peripheral blood lymphocytes and to elucidate initially the mechanisms of immunomodulatory action of these agents. [Methods] The isolated human peripheral blood lymphocytes were stimulated by LPS, co-administrated with or without Amiodarone and Verapamil, respectively. The concentrations of cytokines IL-2, TNF-α, IL-10 and TGF-β1 in collected supernate were detected with ELISA. [Resuits] Amiodarone and Verapamil could inhibit the production of proinflammational cytokines IL-2, TNF-α and anti-inflammation cytokine TGF-β1 in LPS-stimulated human peripheral blood lymphocytes and increase the pro- duction of anti-inflammation cytokine IL-10. [Conclusion] Amiodarone andVerapamil influence the production of cytokines through direct pharmaceutical action on lymphocytes. Ion channels in lymphocytes membrane are possibly one of targets of these two agents.

关 键 词：胺碘酮 维拉帕米 细胞因子 免疫调节 

分 类 号：R392.5[医药卫生—免疫学] R542.2[医药卫生—基础医学]