黄芩苷等中药单体对人皮脂腺细胞内雄性激素受体mRNA表达的影响  

Effects of Baicalin and Other Chinese Herbal Monomer on Androgen Receptor mRNA Expression in SZ95 Sebocytes

作　　者：鞠强[1] 尹兴平[2] 石继海[2] 康晓静[2] 辛燕[2] 夏隆庆[2] 

机构地区：[1]江苏省徐州市第一人民医院皮肤科,江苏徐州221002 [2]中国医学科学院中国协和医科大学皮肤病研究所皮研室,南京210042

出　　处：《中国医学科学院学报》2007年第2期167-170,共4页Acta Academiae Medicinae Sinicae

摘　　要：目的研究盐酸小檗碱、黄芩苷、苦参碱中药单体对SZ95人皮脂腺细胞内雄性激素受体(AR)mRNA表达的影响,探讨中药治疗痤疮的机制。方法应用半定量逆转录聚合酶链反应技术检测不同浓度的盐酸小檗碱、黄芩苷、苦参碱及13-顺维A酸作用SZ95皮脂腺细胞24h后,细胞内ARmRNA的表达。结果1×10-5、1×10-6mol/L13-顺维A酸和1×10-4mol/L黄芩苷使SZ95人皮脂腺细胞中ARmRNA的表达下降(P<0.05)。结论13-顺维A酸和黄芩苷等药物可能通过抑制AR的表达而具有拮抗雄性激素对皮脂腺细胞活性的作用,这将有助于了解这些药物治疗痤疮的机制。Objective To determine the effects of Chinese herbal monomers such as baicalin, berberine, and matrine on the androgen receptor （AR） mRNA expression in SZ95 sebocytes in vitro and to explore the possible mechanism of using traditional Chinese medicines to treat acne. Methods SZ95 sebocytes were cultured and then treated with berberine, baicalin, matrine, and 13-cis-retinoic acid for 24 hours. Reverse transcription polymerase chain reaction was applied to detect the changes of AR. Result AR mRNA was downregulated by 13-cis-retinoic acid of 1 × 10^-5mol/L and 1 × 10^ -6 mol/L, and by baicalin of 1 × 10^ -4 mol/ L （ P 〈 0. 05 ）. Conclusion 13-cis-retinoic acid and baicalin may exert antiandrogenitic action by inhibiting AR mRNA expression in human sebocytes.

关 键 词：盐酸小檗碱 黄芩苷 苦参碱 13-顺维A酸 雄性激素受体 皮脂腺细胞 痤疮 

分 类 号：R758.73[医药卫生—皮肤病学与性病学]