内源性一氧化碳对感染性休克大鼠器官保护作用及机制研究  

Protective effect of endogenous carbon monoxide on organs during septic shock in rat and its mechanisms

作　　者：索晓华[1] 丁春华[1] 谭家庆[1] 黄新莉[1] 凌亦凌[1] 张景坤[1] 

机构地区：[1]河北医科大学病理生理教研室,石家庄050017

出　　处：《中国危重病急救医学》2007年第5期306-310,I0002,共6页Chinese Critical Care Medicine

摘　　要：目的探讨内源性一氧化碳（CO）对感染性休克大鼠肺、肝组织的保护作用及其机制。方法采用盲肠结扎穿孔术（CLP）复制感染性休克模型，按随机数字表法将96只大鼠分为假手术对照组、CLP组、CLP＋氯血红素（Hm）组和CLP＋锌原卟啉（ZnPP）组。各组分别在制模后2、4和6h测定出入肺血中碳氧血红蛋白（COHb）水平；肺、肝组织及血液中丙二醛（MDA）含量及超氧化物歧化酶（SOD）活性；光镜下观察肺、肝组织形态学改变；免疫组化分析血红素加氧酶-1（HO-1）在肺、肝组织中的蛋白表达和分布。结果与假手术对照组比较，CLP组大鼠不同时间点的出入肺血中COHb水平以及肺、肝组织和全血中MDA含量均显著增高（P〈0．05或P〈0．01），SOD活性显著下降（P〈0．05或P〈0．01）；光镜下肺、肝组织损伤严重，HO-1蛋白表达增多。给予Hm后不同时间点，出、入肺血中的COHb水平均较CLP组进一步升高，肺、肝组织及全血中MDA含量均显著下降，SOD活性显著增高；光镜下肺、肝组织损伤明显缓解，HO-1蛋白表达进一步增多。结论感染性休克时内源性CO产生增多可能对肺、肝组织发挥了保护作用。Objective To study the protective effect of endogenous carbon monoxide （CO） on lung and liver during septic shock in rat and its mechanism. Methods Septic shock model was replicated by cecal ligation and puncture （CLP）. Ninety- six rats were randomly divided into sham operation group, CLP group, CLP ＋ hemin （Hm） group and CLP＋zinc protoporphyrin （ZnPP） group. The carboxyhemoglobin （COHb） levels of in - flowing pulmonary blood （IPB） and out - going pulmonary blood （OPB） were determined at 2, 4 and 6 hours after treatments. Malondialdehyde （MDA） contents and superoxide dismutase （SOD） activities in the lung, liver and blood were also determined. Pathological changes in lung and liver were examined with light microscope, and immunohistochemical technique was used for analysis of heme oxygenase - 1 （HO - 1） protein expression and distribution in lung and liver. Results Compared with sham operation group, the COHb level in OPB and IPB as well as MDA contents of lung, liver and blood significantly increased in CLP group, while the SOD activities significantly decreased at differenr time points （P〈0. 05 or P〈0.01）, and the pathological changes and expressions of HO- 1 in two tissues were more marked. However, in CLP＋Hm group the results of MDA, SOD activities and pathological changes were reversed, The content of COHb increased compared to those of CLP group, Immunohistochemical studies showed that there were more HO - 1 postive deposits in CLP ＋ Hm group than those in CLP group. Conclusion Increase in endogenous CO may play a protective role in lung and liver during septic shock.

关 键 词：一氧化碳 休克 感染性 肺脏 肝脏 血红素加氧酶-1 

分 类 号：R686[医药卫生—骨科学]