灵芝孢子能够调节妊高征大鼠所生幼鼠海马线粒体相关分子物质的水平  

Ganoderma spores may regulate the levels of mitochondria-related molecular substances in hippocampus of young rats birthed by rats with gestational hypertension

作　　者：程进军[1] 曾园山[1] 熊轶[1] 张伟 陈穗君[1] 钟志强[1] 

机构地区：[1]中山大学中山医学院组织胚胎学教研室神经科学研究室,广东广州510080

出　　处：《中西医结合学报》2007年第3期322-327,共6页Journal of Chinese Integrative Medicine

摘　　要：目的:探讨灵芝孢子对妊高征大鼠所生幼鼠海马线粒体相关分子物质的影响。方法:用一氧化氮合酶(nitric oxide synthase,NOS)抑制剂N硝基左旋精氨酸甲酯(Nw-nitro-L-arginine methylester,L-NAME)给予大鼠腹腔注射制备妊高征模型,在此基础上胃饲灵芝孢子。应用cAMP放免法、RT-PCR、酶活力检测等技术评价灵芝孢子对妊高征大鼠所生幼鼠海马线粒体相关分子物质水平的影响。结果:妊高征孕鼠的刚分娩幼鼠海马cAMP水平、线粒体DNA(mtDNA)水平及ATP合酶活力都降低。pgc1α表达水平与正常对照大鼠比较没有变化。生后30 d的幼鼠海马cAMP水平、mtDNA水平、ATP合酶活力和pgc1α表达水平低于正常对照大鼠。胃饲灵芝孢子后,妊高征大鼠的刚分娩幼鼠和生后30 d的幼鼠海马cAMP水平和mtDNA含量恢复到正常对照大鼠水平,ATP合酶活力和pgc1α表达水平也有恢复性增高。结论:灵芝孢子具有调节妊高征大鼠所生幼鼠海马线粒体相关分子物质水平的作用。Objective： To investigate the effects of Ganoderma spores on mitochondria-related molecular substances in hippocampus of young rats birthed by rats with gestational hypertension. Methods： Nitric oxide synthase （NOS） inhibitor Nw-nitro-L-arginine methylester （L-NAME） was intraperitoneally injected into pregnant rats to induce gestational hypertension, and Ganoderma spores were administered orally. The effects of Ganoderma spores on levels of mitochondria-related molecular substances in hippocampus of young rats birthed by the rats with gestational hypertension were evaluated with immunoradiometric assay of cAMP, RT-PCR analysis of related genes, and detection of enzyme activity. Results： In hippocampus of the new-born rats birthed by rats with gestational hypertension, the cAMP level, mitochondrial DNA （mtDNA） level and adenosine triphosphatase （ATPase） activity were decreased, and the expression level of peroxisome proliferator activated receptor gamma coactivator 1 alpha （pgclα） was unchanged compared to the normal control group. The cAMP level, mtDNA level, ATPase activity and pgclα expression level in hippocampus of 30-day post-natal rats were lower than those of the rats in normal control group. After oral administration of Ganoderma spores, the cAMP and mtDNA levels in hippocampus of the new-born rats and 30-day post-natal rats recovered almost to the levels of normal control rats, and the ATPase activity and pgclα expression level were also increased significantly. Conclusion. Ganoderma spores may regulate the levels of mitochondria-related molecular substances in hippocampus of young rats birthed by rats with gestational hypertension.

关 键 词：大鼠 灵芝孢子 妊娠高血压 海马 线粒体 线粒体DNA 

分 类 号：R285.5[医药卫生—中药学]