三七总皂苷对大鼠脑缺血再灌注后白细胞介素-1β和其相关因子及半胱氨酸天冬氨酸特异性蛋白酶表达的影响  

Effects of Panax notoginseng saponins on mRNA expressions of interleukin-1β,its correlative factors and cysteinyl-aspartate specific protease after cerebral ischemia-reperfusion in rats

作　　者：唐映红[1] 张淑萍[2] 梁燕[2] 邓常清[2] 

机构地区：[1]湖南中医药大学药学院药理学教研室,湖南长沙410208 [2]湖南中医药大学病理生理学实验室,湖南长沙410007

出　　处：《中西医结合学报》2007年第3期328-332,共5页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金资助项目(No.30171132)

摘　　要：目的:研究三七总皂苷(Panax notoginsengsaponins,PNS)对大鼠脑缺血再灌注后白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-1受体Ⅰ型(interleukin-1 receptor typeⅠ,IL-1RⅠ)、白细胞介素-1受体拮抗剂(interleukin-1 receptor antagonist,IL-1ra)、细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)和半胱氨酸天冬氨酸特异性蛋白酶(cysteinyl-aspartate specific protease,caspase)-1、3、8 mRNA表达的影响。方法:采用颈内动脉线栓法建立大鼠局灶性脑缺血再灌注模型,在脑缺血前5 min和脑缺血后12 h分别腹腔注射给药1次。在脑缺血2 h再灌注22 h后,以逆转录-聚合酶链反应测定缺血脑组织IL-1β、IL-1RⅠ、IL-1ra、ICAM-1和caspase-1、3、8 mRNA的表达。结果:在脑缺血2 h再灌注22 h后,模型组脑组织IL-1β、IL-1RⅠ、IL-1ra、ICAM-1和caspase-1、3、8 mRNA表达较假手术组均增强(P<0.05或P<0.01)。PNS组脑组织IL-1β、IL-1ra和IL-1RⅠ表达低于模型组,高于假手术组,与模型组和假手术组相比,差异均无统计学意义(P>0.05);PNS组脑组织caspase-3表达低于模型组(P<0.05),但PNS对ICAM-1、caspase-1和caspase-8表达无明显影响。结论:PNS可抑制caspase-3的表达,同时对脑缺血后IL-1β及其相关炎症因子的表达有一定抑制作用,从而对缺血性脑损伤发挥保护作用。Objective： To investigate the effects of Panax notoginseng saponins （PNS） on mRNA expressions of interleukin-1β （IL-1β）, interleukin-1 receptor type Ⅰ（IL-1R Ⅰ ）, interleukin-1 receptor antagonist （IL-1ra）, intercellular adhesion molecule-1 （ ICAM-1）, cysteinyl-aspartate specific protease-1 （caspase-1）, caspase-3 and caspase-8 after cerebral ischemia-reperfusion in rats. Methods. Focal cerebral ischemia reperfusion in rats was induced by the method of nylon monofilament via the internal carotid artery. PNS was administered intraperitonealty respectively five minutes before cerebral ischemia and twelve hours after cerebral ischemia. After cerebral ischemia for two hours followed by reperfusion for twenty two hours, the mRNA expressions of IL-1β, IL-1RⅠ, IL-1ra, ICAM-1, caspase-1, caspase-3 and caspase-8 in brain tissue were determined by reverse transcription polymerase chain reaction assav. Results： After cerebral ischemia for two hours followed by reperfusion for twenty two hours, the mRNA expression levels of IL-1β, IL-1RⅠ, IL-1ra, ICAM-1, caspase-1, caspase-3 and caspase-8 in brain tissue in the untreated group were obviously elevated as compared to those in the sham-operation group （ P〈0.05 or P〈0.01）. The mRNA expression levels of IL-1β, IL-1RⅠ, IL-1ra in brain tissue in the PNS group were lower than those in the untreated group, but higher than those in the sham-operation group, and without statistical differences as compared to those in the sham-operation group and in the untreated group （ P〉0.05）. The mRNA expression level of caspase-3 in brain tissue in the PNS group was significantly lower than that in the untreated group （ P〈0.05）, but PNS had no effect on the mRNA expression levels of ICAM-1, caspase-1 and caspase-8 in brain tissue. Conclusion： PNS can inhibit the mRNA expression of caspase-3, slightly inhibit the mRNA expressions of IL-1β and its correlative inflammatory factors in brain tissue. The protective effects of PNS on cer

关 键 词：脑缺血 再灌注 白细胞介素-1Β 白细胞介素-1 白细胞介素-1受体 半胱氨酸天冬氨酸特异性蛋白酶 逆转录聚合酶链反应 大鼠 

分 类 号：R285.5[医药卫生—中药学]