正己烷慢性吸入小鼠实验模型建立  

Mice model etablishment of n-hexane chronic inhalation in mice

作　　者：张大勇[1] 黄中新[1] 曹静婷[1] 程欣[1] 陈瑛[2] 

机构地区：[1]暨南大学医学院,广州510632 [2]广州市第十二人民医院

出　　处：《中国公共卫生》2007年第5期590-591,共2页Chinese Journal of Public Health

基　　金：广东省医学科研基金(A2006566);广州市医药卫生科技项目(2005-YB-102)

摘　　要：目的建立正己烷慢性吸入染毒的动物实验模型,为研究正己烷长期吸入的毒理损伤机制提供实验方法。方法24只昆明小鼠,随机分为4组(正常对照组、染毒4,8和12周组),静式染毒,初始浓度为17.6g/m3。染毒结束后,取血标本测正己烷浓度;取肺、肝和肾组织常规固定,包埋,光镜观察结构变化。结果各染毒组小鼠血液中正己烷平均浓度均已明显高于正常对照组,已达到染毒要求。镜下观察实验小鼠的肺、肝和肾均出现病理变化,并随染毒时间延长呈病理改变加重趋势。结论本实验采用的昆明小鼠正己烷静式染毒方法,已造成实验小鼠主要代谢器官进行性损伤,可作为研究正己烷毒理损伤机制的参考模型。Objective To establish a simple and feasible mice model of n-hexane long-term inhalation for investigating the mechanism of toxicity of n-hexane. Methods 24 healthy mice were randomly divided into 4 groups： control group, poisoning groups（ 4w, 8w and 12 w）. Primary concentration of n-hexane was 17.6g/m^3. At the end of the experiment, the nhexane concentration of blood was detected, some fresh tissues were prepared for paraffin imbedding and HE staining. Results The mice of poisoning groups were found less activity and weight. The poisoning groups＇ average blood concentrations of n-hexane were higher markedly than that of the control group, and there were some progressive pathologic damage in lungs, livers and kidneys in poisoning groups. Conclusion This mice model of n-hexane long-term inhalation is useful and feasible to study the mechanism of toxicity of n-hexane.

关 键 词：正己烷 昆明小鼠 静式染毒 动物模型 

分 类 号：R135.1[医药卫生—劳动卫生]