RP-HPLC determination of lycopene in microcapsules  

RP-HPLC测定番茄红素微胶囊的含量(英文)

作　　者：卢定强[1] 陆晓云[1] 陆凯丰[1] 胡继军[1] 

机构地区：[1]南京工业大学制药与生命科学学院,江苏南京210009

出　　处：《Journal of Chinese Pharmaceutical Sciences》2007年第1期65-68,共4页中国药学（英文版）

基　　金：Special Research Foundation of Ph.D. Study in University(20040291004);Major Project of Chinese(National Programs for Fundamental Research(2003CB716000)

摘　　要：Aim A RP- HPLC method for determination of lycopene in microcapsules was established. Methods The HPLC assay was performed on an Alltima Cls （4.6 mm × 250 mm, 5μm） column with a mixture of methanol-THF-water （66：30：4, V/V/V） as mobile phase at a flow rate of 1.5 mL·min^-1 and the UV detection wavelength was 472 nm. Results The linear range of lycopene was 3.6-18 μg·mL^-1, r = 0.999 8, the average recovery was from 99.81% to 101.06% with RSD less than 1.83%. The RSD of intra-day and interday precision were less than 3.34%. Conclusion The method is simple, accurate and suitable for the determination of lycopene in microcapsules.目的建立反相高效液相色谱法测定番茄红素微胶囊中番茄红素含量的方法。方法采用反相高效液相色谱法,色谱柱为美国Alltech Alltima C1(84.6mm×250mm,5μm),流动相为甲醇-四氢呋喃-水(66∶30∶4,V/V/V),流速为1.5mL·min-1,检测波长为472nm。结果线性范围为 3.6～18μg·mL-1,相关系数r=0.9998,平均回收率为99.81%～101.06%,RSD为1.12%～1.83%,日内和日间精密度的RSD分别在1.73%～2.54%、2.85%～3.34%之间。结论本文采用反相高效液相色谱法初步建立了番茄红素微胶囊中番茄红素的定量方法, 该方法准确, 快速, 重现性好。

关 键 词：RP-HPLC LYCOPENE MICROCAPSULES 

分 类 号：R927[医药卫生—药学]