不同腐植酸原料中的微生物分离与鉴定(上)  

Isolation and Identif ication of Microbes from Different Humic Acid Materials (Ⅰ)

作　　者：肖晶晶[1] 郭萍[1] 田云龙[1] 于江[1] 叶婧[1] 朱昌雄[1] 

机构地区：[1]中国农业科学院农业环境与可持续发展研究所,北京100081

出　　处：《腐植酸》2007年第2期9-14,共6页Humic Acid

基　　金：农业部结构调整项目(06-08-02A);国家自然科技资源平台项目(2005DKA21201)

摘　　要：从32种不同产地、不同种类的腐植酸原料中分离微生物,以验证腐植酸原料中是否存在微生物;如果存在,则对其进行菌种鉴定。目前,关于腐植酸中存在微生物的研究鲜有报道。但是腐植酸中可能会存在一些有益的微生物,对其进行研究,可以为以后研究腐植酸与微生物两者之间的相互作用及应用奠定基础。试验结果表明,腐植酸原料中(除上海生化腐植酸外)存在细菌、放线菌、真菌,并且不同腐植酸中微生物的种类、数量不同,但优势菌大致相同;从中分离得到细菌优势菌8株、放线菌优势菌4株、真菌优势菌5株,通过菌落形态、菌体形态以及生理生化特征等方面鉴定各菌种。鉴定结果明确,它们属于短小杆菌属(Curtobacte-rium Yamada etc.,1972)、芽孢杆菌属的短小芽孢杆菌(B.pumilus)、灰褐类群阿拉伯链霉菌(S.arabicus Shibata etc.,1957)等。In this paper, the microbial characteristics of 32 kinds of humic acid materials from different regions were studied. At present, there are few reports about the study whether there are microbes in humic acids. Maybe there are, so we study it, and it will make a base for the next study. The results showed that bacteria, actinomyces and fungi were all in humic acid materials except for Shanghai biochemical HA, and the species and number of microbe in different humic acid materials were different, but doininent microbe was the same. Meantime, 10 dominant strains of bacteria, 4 dominant strains of actinomyces, and 5 dominant strains of fungi were isolated. According to colonial morphology, mycelial morphology, and physiological-biochemical characteristics, the microbes were identified as Curtobacterium Yamada, B.pumilus, S.arabicus Shibata, 1957 and so on.

关 键 词：腐植酸原料 微生物 菌种分离鉴定 

分 类 号：TQ440.1[化学工程—化学肥料工业]