苦参碱对细菌脂多糖诱导大鼠枯否细胞释放肿瘤坏死因子及白细胞介素-6的影响  

INHIBITORY EFFECT OF MATRINE ON LIPOPOLYSACCHRIDE INDUCED TUMOR NECROSIS FACTOR AND INTERLEUKIN 6 PRODUCTION FROM RAT KUPFFER CELLS

作　　者：林文[1] 张俊平[1] 胡振林[1] 钱定华[1] 

机构地区：[1]第二军医大学药学院中西药研究室

出　　处：《药学学报》1997年第2期93-96,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金

摘　　要：目的是研究苦参碱对细菌脂多糖（ｌｉｐｏｐｏｌｙｓａｃｈｒｉｄｅｓ，ＬＰＳ）诱导经卡西霉素（ｃａｌｃｉｍｙｃｉｎ，Ｃａｌ）预激活的大鼠枯否细胞分泌肿瘤坏死因子（ｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒ，ＴＮＦ）、白细胞介素６（ｉｎｔｅｒｌｅｕｋｉｎ６，ＩＬ６）的影响以及对小鼠体内产生ＴＮＦ和ＩＬ６的影响。结果，苦参碱１２５，２５０及５００ｍｇ·Ｌ－１剂量依赖性抑制大鼠枯否细胞分泌ＴＮＦ和ＩＬ６；苦参碱５０及１００ｍｇ·ｋｇ－１降低小鼠体内ＴＮＦ和ＩＬ６的水平。提示苦参碱的抗炎作用可能与其抑制ＴＮＦ及ＩＬ６的产生有关。The effect of matrine on the lipopolysacchride (LPS) induced tumor necrosis factor and interleukin 6 production from rat Kupffer cell was investigated. Results showed that matrine 125, 250 and 500 mg·L -1 suppressed TNF and IL 6 production from Cal primed Kupffer cells in the presence of lipopolysacchrides (LPS, 100 μg·L -1 ) in a concentration dependent manner. Treatment with matrine 50 and 100 mg·kg -1 before LPS injection(3.5 mg·kg -1 ) markedly decreased mouse serum TNF and IL 6. The results suggest that matrine may have protective effect on LPS inuced liver injury.

关 键 词：苦参碱 枯否细胞 TNF 白细胞介素6 细菌脂多糖 

分 类 号：R965[医药卫生—药理学]